Localization of l -DOPA uptake and decarboxylating neuronal structures in the cat brain using dopamine immunohistochemistry

Abstract The present study examined dopamine-immunoreactive neuronal structures using immunohistochemistry in conjunction with an anti-dopamine antiserum, following injection of l -3,4-dihydroxyphenylalanine ( l -DOPA) with or without an inhibitor of monoamine oxidase (Pargyline) in the cat brain. l...

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Bibliographic Details
Published in:Brain research 2007-09, Vol.1167, p.56-70
Main Authors: Kitahama, Kunio, Geffard, Michel, Araneda, Silvia, Arai, Ryohachi, Ogawa, Keiko, Nagatsu, Ikuko, Pequignot, Jean-Marc
Format: Article
Language:English
Subjects:
Animals
Aromatic l-amino acid decarboxylase
Aromatic-L-Amino-Acid Decarboxylases
Aromatic-L-Amino-Acid Decarboxylases - metabolism
Axons
Axons - metabolism
Brain
Brain - anatomy & histology
Brain - metabolism
Cats
Dopamine
Dopamine - biosynthesis
Immunohistochemistry
Immunohistochemistry - methods
l-3,4-dihydroxyphenylalanine
Levodopa
Levodopa - metabolism
Levodopa - pharmacology
Life Sciences
Monoamine oxidase
Monoamine Oxidase Inhibitors
Monoamine Oxidase Inhibitors - pharmacology
Neurochemistry
Neurochemistry - methods
Neurology
Neurons
Neurons - metabolism
Norepinephrine
Norepinephrine - metabolism
Other
Pargyline
Serotonin
Serotonin - metabolism
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Summary:Abstract The present study examined dopamine-immunoreactive neuronal structures using immunohistochemistry in conjunction with an anti-dopamine antiserum, following injection of l -3,4-dihydroxyphenylalanine ( l -DOPA) with or without an inhibitor of monoamine oxidase (Pargyline) in the cat brain. l -DOPA injection made it possible to detect dopamine immunoreactivity in presumptive serotonergic and noradrenergic cell bodies and axons. Weak to moderate dopamine immunoreactivity was observed in non-aminergic cells (possibly so-called “D” cells containing aromatic l -amino acid decarboxylase (AADC)) in several hypothalamic, midbrain, pontine and medullary nuclei. Intense dopamine immunoreactivity became visible in a large number of cells and axons (possibly containing AADC) with wide distribution in the brain following administration of l -DOPA with Pargyline. AADC is most likely active in cells and axons that take up l -DOPA, where it decarboxylates the l -DOPA to dopamine. However, newly synthesized dopamine in such cells is rapidly oxidized by monoamine oxidase.
ISSN:0006-8993
1872-6240
0006-8993
DOI:10.1016/j.brainres.2007.05.081