Control of gcm RNA stability is necessary for proper glial cell fate acquisition

The control of RNA stability is an important post-transcriptional event. While neural development is known to require proteins that bind AU-rich elements (ARE) and affect RNA half-life, the role of specific RNA stability in this process remains elusive. In the Drosophila embryo, glial fate acquisiti...

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Bibliographic Details
Published in:Molecular and cellular neuroscience 2008-04, Vol.37 (4), p.657-662
Main Authors: Soustelle, Laurent, Roy, Nivedita, Ragone, Gianluca, Giangrande, Angela
Format: Article
Language:English
Subjects:
3' Untranslated Regions - genetics
Adenosine - genetics
Animals
Base Sequence
Biochemistry, Molecular Biology
DNA-Binding Proteins - genetics
DNA-Binding Proteins - physiology
Drosophila
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Drosophila Proteins - physiology
Glide/Gcm
Gliogenesis
Life Sciences
Molecular biology
Molecular Sequence Data
Neuroglia - cytology
Neuroglia - physiology
RNA decay
RNA Stability - genetics
Transcription Factors - genetics
Transcription Factors - physiology
Uridine - genetics
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Summary:The control of RNA stability is an important post-transcriptional event. While neural development is known to require proteins that bind AU-rich elements (ARE) and affect RNA half-life, the role of specific RNA stability in this process remains elusive. In the Drosophila embryo, glial fate acquisition is triggered by glial cells missing ( gcm) master gene, which is transiently expressed in all gliogenic stem cells and submitted to tight transcriptional regulation. By using in vitro and in vivo site directed mutagenesis, we have discovered that gcm RNA is unstable and that its 3′UTR confers labile properties to RNA due to the presence of an ARE motif. Moreover, we show that the gliogenic potential of Gcm transcription factor increases when ARE is abolished and demonstrate the importance of gcm RNA stability in the acquisition of the glial fate. Thus, control of a single RNA half-life is crucial for nervous system differentiation.
ISSN:1044-7431
1095-9327
DOI:10.1016/j.mcn.2007.11.007