The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure

Abstract Cell adhesion and migration are strongly influenced by extracellular matrix (ECM) architecture and rigidity, but little is known about the concomitant influence of such environmental signals to cell responses, especially when considering cells of similar origin and morphology, but exhibitin...

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Bibliographic Details
Published in:Biomaterials 2008-04, Vol.29 (10), p.1541-1551
Main Authors: Tzvetkova-Chevolleau, Tzvetelina, Stéphanou, Angélique, Fuard, David, Ohayon, Jacques, Schiavone, Patrick, Tracqui, Philippe
Format: Article
Language:English
Subjects:
Advanced Basic Science
Algorithms
Animals
Biomechanical Phenomena
Biomechanics
Cell Adhesion
Cell Adhesion - drug effects
Cell Behavior
Cell Line, Tumor
Cell Movement
Cellular Biology
Dentistry
Dimethylpolysiloxanes
Dimethylpolysiloxanes - chemistry
Dimethylpolysiloxanes - pharmacology
Engineering Sciences
Extracellular Matrix
Extracellular Matrix - chemistry
Extracellular Matrix - ultrastructure
Kinetics
Life Sciences
Mechanotaxis
Mice
Micro and nanotechnologies
Microelectronics
Micropatterning
Microscopy, Electron, Scanning
NIH 3T3 Cells
Random walk
Silicones
Silicones - chemistry
Silicones - pharmacology
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Summary:Abstract Cell adhesion and migration are strongly influenced by extracellular matrix (ECM) architecture and rigidity, but little is known about the concomitant influence of such environmental signals to cell responses, especially when considering cells of similar origin and morphology, but exhibiting a normal or cancerous phenotype. Using micropatterned polydimethylsiloxane substrates (PDMS) with tunable stiffness (500 kPa, 750 kPa, 2000 kPa) and topography (lines, pillars or unpatterned), we systematically analyse the differential response of normal (3T3) and cancer (SaI/N) fibroblastic cells. Our results demonstrate that both cells exhibit differential morphology and motility responses to changes in substrate rigidity and microtopography. 3T3 polarisation and spreading are influenced by substrate microtopography and rigidity. The cells exhibit a persistent type of migration, which depends on the substrate anisotropy. In contrast, the dynamic of SaI/N spreading is strongly modified by the substrate topography but not by substrate rigidity. SaI/N morphology and migration seem to escape from extracellular cues: the cells exhibit uncorrelated migration trajectories and a large dispersion of their migration speed, which increases with substrate rigidity.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2007.12.016