Changes in plasma triacylglycerol concentrations after sequential lunch and dinner in healthy subjects

• Published in: Diabetes & metabolism 2005-02, Vol.31 (1), p.69-77
• Main Authors: Maillot, F, Garrigue, MA, Pinault, M, Objois, M, Théret, V, Lamisse, F, Hoinard, C, Antoine, JM, Lairon, D, Couet, C
• Format: Article
• Language: English
• Subjects: [1 13C] palmitate; Adult; Biological and medical sciences; Chylomicron; Circadian Rhythm; Dietary fat; Dietary Fats; domain_sdv.aen.nut; Food and Nutrition; Fundamental and applied biological sciences. Psychology; Graisses alimentaires; Humans; Life Sciences; Lipids. Glycolipids; Lipémie postprandiale; Male; Metabolisms and neurohumoral controls; Olive Oil; Plant Oils; Postprandial lipemia; Postprandial Period; Reference Values; Sunflower Oil; Triacylglycerol; Triglycerides - blood; Triglycérides; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 1262-3636; 1878-1780
• DOI: 10.1016/S1262-3636(07)70169-6

The present study examines the kinetic of plasma triacylglycerol (TAG) after sequential ingestion of lunch and dinner as well as the contribution of dietary fat ingested at lunch to subsequent post-dinner TAG composition. Six healthy subjects were included. After standardized breakfast (7: 30AM), 2 mixed meals with fat loads composed of 44 g olive oil (rich in oleic acid) at lunch (12AM) and 44 g sunflower oil (rich in linoleic acid) at dinner (7PM) were ingested. [1- 13C] palmitate was added in lunch only. Plasma TAG and chylomicron-TAG (CMTAG) levels were measured sequentially after meals. [1- 13C] palmitate enrichment and concentrations of oleic acid and linoleic acid were measured in all lipid fractions. Post-dinner plasma TAG peak was delayed as compared to lunch (3 hours vs 1 hour, p = 0.002) whereas the magnitude of the postprandial peaks was not significantly different between lunch and dinner (2.4 ± 0.3 vs 2.0 ± 0.4 mmol/L, p = 0.85). [1- 13C] palmitate enrichment was maximal 5 hours after lunch in all lipid fractions and decreased slowly thereafter. After dinner ingestion, the rate of decline of [1- 13C] palmitate enrichment plateaued during the first 60 minutes. Oleic acid increased slightly and immediately after dinner and remained the predominant fatty acid in all lipid fractions during the first hour after dinner. A delayed peak of plasma and CM-TAG was observed after dinner as compared to lunch without difference in the magnitude of peaks. The contribution of dietary fat ingested at lunch to post-dinner lipemia is confirmed despite the relatively long lasting interval between the 2 meals (7 h) and the absence of any early peak of plasma TAG after dinner. Modifications des concentrations de triglycérides plasmatiques après prise séquentielle d'un déjeuner et d'un dîner chez des sujets sains Cette étude examine la cinétique des triglycérides plasmatiques (TAG) après ingestion successive d'un déjeuner et d'un dîner, ainsi que la contribution des graisses ingérées au déjeuner à la lipémie postprandiale du dîner. Six sujets sains furent inclus. Après un petit déjeuner standardisé (7 h 30), 2 repas mixtes ont été ingérés, avec des charges en graisses de 44 g d'huile d'olive (riche en oléate) au déjeuner (12 h 00) et 44 g d'huile de tournesol (riche en linoléate) au dîner (19 h 00). Du [1- 13C] palmitate était ajouté uniquement au déjeuner. Les concentrations de TAG et triglycérides des chylomicrons (CMTAG) étaient régulièrement mesurées après les