Daily intake of conjugated linoleic acid-enriched yoghurts: effects on energy metabolism and adipose tissue gene expression in healthy subjects

• Published in: British journal of nutrition 2007-02, Vol.97 (2), p.273-280
• Main Authors: Nazare, Julie-Anne, Perrière, Aude Brac de la, Bonnet, Fabrice, Desage, Michel, Peyrat, Jocelyne, Maitrepierre, Christine, Louche-Pelissier, Corinne, Bruzeau, Joëlle, Goudable, Joëlle, Lassel, Taous, Vidal, Hubert, Laville, Martine
• Format: Article
• Language: English
• Subjects: Acids; Adipocytes; Adipose tissue; Adipose Tissue - physiology; Adipose tissue gene expression; Adult; adults; animal proteins; basal metabolism; Biological and medical sciences; Biopsy; Blood Glucose - analysis; Body composition; Body Composition - physiology; Body fat; Body mass index; Body weight; carboxylic ester hydrolases; Conjugated linoleic acid; Dairy products; Diet; Dietary Supplements; domain_sdv.ida.ing; domain_sdv.ida.ing.lip; Double-Blind Method; Energy; Energy expenditure; Energy metabolism; Energy Metabolism - physiology; enriched foods; Enzymes; fat free mass; Fatty acids; Fatty Acids - analysis; Feeding. Feeding behavior; Female; Food engineering; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression - physiology; Glucose; Health; hormone-sensitive lipase; Humans; Insulin - blood; Ion Channels - genetics; Isomers; Leptin - blood; Life Sciences; Linoleic acid; Linoleic Acids, Conjugated - administration & dosage; Lipase; Lipids; Lipids - blood; Lipoprotein lipase; Lipoprotein Lipase - genetics; Male; Metabolism; Mitochondrial Proteins - genetics; Mitochondrial uncoupling protein 2; Nitrogen; Nutrition; Nutrition research; Obesity; Overweight; Oxidation; Peroxisome proliferator-activated receptors; Plasma; PPAR gamma - genetics; Sterol Esterase - genetics; Studies; Tissues; transcription factors; triacylglycerols; Triglycerides; Uncoupling Agents; Uncoupling Protein 2; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Yogurt
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114507191911

Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of conjugated dienoic derivatives of linoleic acid. The present study was designed to determine whether 14-week CLA supplementation as triacylglycerols (3·76 g) with a 50 : 50 combination of the two main isomers (35 % cis-9, trans-11 and 35 % trans-10, cis-12) added to flavoured yoghurt-like products was able to alter body composition in healthy subjects and to alter the expression of several key adipose tissue genes (PPAR γ, lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and uncoupling protein 2 (UCP-2)). Forty-four healthy subjects were randomly assigned to consume daily either a CLA-supplemented yoghurt-like product or a placebo yoghurt for 98 d. There were no significant effects of CLA supplementation on body weight, fat mass or free fat mass. Basal energy expenditure expressed as kg free fat mass increased significantly in the CLA group (123·3 (sem 2·5) kJ/kg free fat mass per d on day 98 v. 118·7 (sem 2·3) kJ/kg free fat mass per d on day 0, P = 0·03). PPAR γ mRNA gene expression increased significantly with CLA supplementation (53 (sem 20) %, P