Identification of glucuronan lyase from a mutant strain of Rhizobium meliloti

The Rhizobium meliloti M5N1CS (NCIMB 40472) mutant strain wich induces nodule formation on alfalfa roots, produces a (1→4)- β- d-glucuronan partially acetylated. During fermentation under specific conditions, the molecular weight of the polymer decrease, the presence of polysaccharide degrading enzy...

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Bibliographic Details
Published in:International journal of biological macromolecules 1997-08, Vol.21 (1), p.3-9
Main Authors: Michaud, P, Pheulpin, P, Petit, E, Séguin, J.P, Barbotin, J.N, Heyraud, A, Courtois, B, Courtois, J
Format: Article
Language:English
Subjects:
Acetylation
Carbohydrate Metabolism
Carbohydrates - chemistry
Chromatography - methods
Glucuronan lyase
Magnetic Resonance Spectroscopy
Mutation
Polysaccharide
Polysaccharide-Lyases - chemistry
Polysaccharide-Lyases - metabolism
Polysaccharides - chemistry
Polysaccharides - metabolism
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - metabolism
Rhizobium meliloti
Sinorhizobium meliloti - enzymology
Sinorhizobium meliloti - genetics
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Summary:The Rhizobium meliloti M5N1CS (NCIMB 40472) mutant strain wich induces nodule formation on alfalfa roots, produces a (1→4)- β- d-glucuronan partially acetylated. During fermentation under specific conditions, the molecular weight of the polymer decrease, the presence of polysaccharide degrading enzyme was suspected. A glucuronan lyase was identified, this new bacterial lyase produces d.p. 4 oligoglucuronans, substituents (acetates) present on the substrate reduced the enzyme activity.
ISSN:0141-8130
1879-0003
DOI:10.1016/S0141-8130(97)00033-0