Structural aspects of fish skin collagen which forms ordered arrays via liquid crystalline states

The ability of acid-soluble type I collagen extracts from Soleidae flat fish to form ordered arrays in condensed phases has been compared with data for calf skin collagen. Liquid crystalline assemblies in vitro are optimized by preliminary treatment of the molecular population with ultrasounds. This...

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Bibliographic Details
Published in:Biomaterials 2000-05, Vol.21 (9), p.899-906
Main Authors: Giraud-Guille, Marie-Madeleine, Besseau, Laurence, Chopin, Christine, Durand, Patrick, Herbage, Daniel
Format: Article
Language:English
Subjects:
Animals
Biochemistry, Molecular Biology
Biological and medical sciences
Biomaterials
Calorimetry, Differential Scanning
Cattle
Collagen
Collagen - chemistry
Collagen - ultrastructure
Crystalline structure
Crystallization
Differential scanning calorimetry
Electrophoresis
Fish skin
Fishes
Fundamental and applied biological sciences. Psychology
Life Sciences
Liquid crystals
Microscopy, Electron
Molecular biophysics
Phase composition
Protein Conformation
Skin
Skin - chemistry
Skin - ultrastructure
Structure in molecular biology
Three-dimensional matrices
Ultrasonic applications
X ray diffraction analysis
X-Ray Diffraction
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Summary:The ability of acid-soluble type I collagen extracts from Soleidae flat fish to form ordered arrays in condensed phases has been compared with data for calf skin collagen. Liquid crystalline assemblies in vitro are optimized by preliminary treatment of the molecular population with ultrasounds. This treatment requires the stability of the fish collagen triple helicity to be controlled by X-ray diffraction and differential scanning calorimetry and the effect of sonication to be evaluated by viscosity measurements and gel electrophoresis. The collagen solution in concentrations of at least 40 mg ml −1 showed in polarized light microscopy birefringent patterns typical of precholesteric phases indicating long-range order within the fluid collagen phase. Ultrastructural data, obtained after stabilization of the liquid crystalline collagen into a gelated matrix, showed that neutralized acid-soluble fish collagen forms cross-striated fibrils, typical of type I collagen, following sine wave-like undulations in precholesteric domains. These ordered geometries, approximating in vivo situations, give interesting mechanical properties to the material.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(99)00244-6