Type IV collagen in sponges, the missing link in basement membrane ubiquity

Basement membrane structures, or their main component, type IV collagen, have been detected in all multicellular animal species, except sponges. We cancel this exception by the demonstration of type IV collagenous sequences in a new marine sponge species by cDNA and genomic DNA studies. One of these...

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Bibliographic Details
Published in:Biology of the cell 1996, Vol.88 (1), p.37-44
Main Authors: Boute, Nicolas, Exposito, Jean-Yves, Boury-Esnault, Nicole, Vacelet, Jean, Noro, Nobuhiro, Miyazaki, Koyomi, Yoshizato, Katsutoshi, Garrone, Robert
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Basement Membrane - chemistry
basement membranes
Biochemistry, Molecular Biology
Cloning, Molecular
collagen
Collagen - analysis
cysteine
DNA, Complementary - isolation & purification
evolution
Genome
Humans
Life Sciences
Marine
Molecular Sequence Data
Oligonucleotide Probes
Phylogeny
Porifera
Porifera - chemistry
porifere
Pseudocorticum jarrei
Species Specificity
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Summary:Basement membrane structures, or their main component, type IV collagen, have been detected in all multicellular animal species, except sponges. We cancel this exception by the demonstration of type IV collagenous sequences in a new marine sponge species by cDNA and genomic DNA studies. One of these sequences is long enough to demonstrate the specific characteristics of type IV collagen chains. The 12 cysteines are at conserved positions in the carboxyl-terminal non-helical NC1 domain, as are the interruptions in the carboxyl-terminal end of the triple helical domain. The gene organization of the region coding for the NC1 domain is similar to that of the human genes COL4A2, COL4A4 and COL4A6. An additional, shorter sequence suggests the presence of a second chain. The expected tissue localization of this collagen has been confirmed using polyclonal antibodies raised against a sponge recombinant protein. These results demonstrate that type IV collagen is representated in all animal phyla. It is actually the only known ubiquitous collagen and it has at least two different alpha chains in all the species where it has been characterized.
ISSN:0248-4900
1768-322X
DOI:10.1016/S0248-4900(97)86829-3