Detection of exogenous intake of natural corticosteroids by gas chromatography/combustion/isotope ratio mass spectrometry: application to misuse in sport

A detailed procedure for the analysis of exogenous hydrocortisone and cortisone in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is proposed. As urinary levels of hydrocortisone are rather low for GC/C/IRMS analysis, the focus is on the main corticosteroid metabo...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2000-01, Vol.14 (24), p.2343-2347
Main Authors: Bourgogne, Emmanuel, Herrou, Valérie, Mathurin, Jean-Charles, Becchi, Michel, Ceaurriz, Jacques de
Format: Article
Language:English
Subjects:
Adrenal Cortex Hormones - administration & dosage
Adrenal Cortex Hormones - chemistry
Adrenal Cortex Hormones - metabolism
Adrenal Cortex Hormones - urine
Adult
Androstanes - chemistry
Androstanes - urine
Biochemistry, Molecular Biology
Cortisone - administration & dosage
Cortisone - chemistry
Cortisone - metabolism
Cortisone - urine
Female
Gas Chromatography-Mass Spectrometry - methods
Humans
Hydrocortisone - administration & dosage
Hydrocortisone - chemistry
Hydrocortisone - metabolism
Hydrocortisone - urine
Life Sciences
Male
Middle Aged
Molecular Structure
Sports
Tetrahydrocortisol - chemistry
Tetrahydrocortisol - urine
Tetrahydrocortisone - chemistry
Tetrahydrocortisone - urine
Time Factors
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Summary:A detailed procedure for the analysis of exogenous hydrocortisone and cortisone in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is proposed. As urinary levels of hydrocortisone are rather low for GC/C/IRMS analysis, the focus is on the main corticosteroid metabolites, tetrahydrocortisone (THE) and tetrahydrocortisol (THF). Following different solid phase extraction purifications, THE and THF are oxidized to 5β‐androstanetrione before analysis by GC/C/IRMS. Significant differences in δ13C‰ values of synthetic natural corticosteroids and endogenous human corticosteroids have been observed. Therefore, a positive criterion, to detect exogenous administration of synthetic corticosteroids in anti‐doping control, is proposed. Copyright © 2000 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/1097-0231(20001230)14:24<2343::AID-RCM171>3.0.CO;2-Z