Revisiting TRPC1 and TRPC6 mechanosensitivity

This article addresses whether TRPC1 or TRPC6 is an essential component of a mammalian stretch-activated mechano-sensitive Ca 2+ permeable cation channel (MscCa). We have transiently expressed TRPC1 and TRPC6 in African green monkey kidney (COS) or Chinese hamster ovary (CHO) cells and monitored the...

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Bibliographic Details
Published in:Pflügers Archiv 2008-03, Vol.455 (6), p.1097-1103
Main Authors: Gottlieb, Philip, Folgering, Joost, Maroto, Rosario, Raso, Albert, Wood, Thomas G., Kurosky, Alex, Bowman, Charles, Bichet, Delphine, Patel, Amanda, Sachs, Frederick, Martinac, Boris, Hamill, Owen P., Honoré, Eric
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Membrane
Cell Membrane - metabolism
Cells
Cercopithecus aethiops
CHO Cells
COS Cells
Cricetinae
Cricetulus
Human health and pathology
Human Physiology
Humans
Ion Channels
Life Sciences
Mechanoreceptors
Mechanoreceptors - physiology
Microscopy, Confocal
Molecular Medicine
Neurons and Cognition
Neurosciences
Patch-Clamp Techniques
Pharmaceutical sciences
Plasmids
Receptors
Transfection
TRPC Cation Channels
TRPC Cation Channels - biosynthesis
TRPC Cation Channels - genetics
TRPC Cation Channels - physiology
TRPC6 Cation Channel
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Summary:This article addresses whether TRPC1 or TRPC6 is an essential component of a mammalian stretch-activated mechano-sensitive Ca 2+ permeable cation channel (MscCa). We have transiently expressed TRPC1 and TRPC6 in African green monkey kidney (COS) or Chinese hamster ovary (CHO) cells and monitored the activity of the stretch-activated channels using a fast pressure clamp system. Although both TRPC1 and TRPC6 are highly expressed at the protein level, the amplitude of the mechano-sensitive current is not significantly altered by overexpression of these subunits. In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic.
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-007-0359-3