The effect of stromelysin-1 (MMP-3) on non-collagenous extracellular matrix proteins of demineralized dentin and the adhesive properties of restorative resins

Abstract Dentin non-collagenous matrix components (NCPs) are structural proteins involved in the formation, the architecture and the mineralization of the extracellular matrix (ECM). We investigated here how recombinant metalloproteinase stromelysin-1, also termed MMP-3, initiates the release of ECM...

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Bibliographic Details
Published in:Biomaterials 2008-11, Vol.29 (33), p.4367-4373
Main Authors: Boukpessi, T, Menashi, S, Camoin, L, TenCate, J.M, Goldberg, M, Chaussain-Miller, C
Format: Article
Language:English
Subjects:
Adhesion
Adhesives - metabolism
Adolescent
Advanced Basic Science
Cellular Biology
Child
Dentin
Dentin - chemistry
Dentin - metabolism
Dentin - ultrastructure
Dentistry
Extracellular matrix proteins (ECM)
Extracellular Matrix Proteins - genetics
Extracellular Matrix Proteins - metabolism
Humans
Integrin-Binding Sialoprotein
Life Sciences
Matrix Metalloproteinase 3 - analysis
Matrix Metalloproteinase 3 - genetics
Matrix Metalloproteinase 3 - metabolism
Molecular Weight
Osteopontin - analysis
Osteopontin - chemistry
Osteopontin - metabolism
Proteoglycans
Proteoglycans - chemistry
Proteoglycans - metabolism
Recombinant Proteins - metabolism
Resins, Synthetic - metabolism
Sialoglycoproteins - chemistry
Sialoglycoproteins - metabolism
Stromelysin-1 (MMP-3)
Time Factors
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Summary:Abstract Dentin non-collagenous matrix components (NCPs) are structural proteins involved in the formation, the architecture and the mineralization of the extracellular matrix (ECM). We investigated here how recombinant metalloproteinase stromelysin-1, also termed MMP-3, initiates the release of ECM molecules from artificially demineralized human dentin. Analysis of the supernatants by Western blotting reveals that MMP-3 extracts PGs (decorin, biglycan), and also a series of phosphorylated proteins: dentin sialoprotein (DSP), osteopontin (OPN), bone sialoprotein (BSP) and MEPE, but neither dentin matrix protein-1 (DMP1), another member of the SIBLING family, nor osteocalcin (OC), a non-phosphorylated matrix molecule. After treatment of dentin surfaces by MMP-3, scanning electron microscope (SEM) examination of resin replica shows an increased penetration of the resin into the dentin tubules when compared to surfaces only treated by demineralizing solutions. This preclinical investigation suggests that MMP-3 may be used to improve the adhesive properties of restorative materials.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2008.07.035