High-Resolution Intracellular Recordings Using a Real-Time Computational Model of the Electrode

Intracellular recordings of neuronal membrane potential are a central tool in neurophysiology. In many situations, especially in vivo, the traditional limitation of such recordings is the high electrode resistance and capacitance, which may cause significant measurement errors during current injecti...

Full description

Saved in:
Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2008-08, Vol.59 (3), p.379-391
Main Authors: Brette, Romain, Piwkowska, Zuzanna, Monier, Cyril, Rudolph-Lilith, Michelle, Fournier, Julien, Levy, Manuel, Frégnac, Yves, Bal, Thierry, Destexhe, Alain
Format: Article
Language:English
Subjects:
Animals
Bias
Computer Simulation
Electrodes
Estimates
Life Sciences
Membrane Potentials
Membrane Potentials - physiology
Microelectrodes
MOLNEURO
Neurons
Neurons - physiology
Neurons and Cognition
Neurophysiology
Neurophysiology - instrumentation
Patch-Clamp Techniques
Patch-Clamp Techniques - methods
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Intracellular recordings of neuronal membrane potential are a central tool in neurophysiology. In many situations, especially in vivo, the traditional limitation of such recordings is the high electrode resistance and capacitance, which may cause significant measurement errors during current injection. We introduce a computer-aided technique, Active Electrode Compensation (AEC), based on a digital model of the electrode interfaced in real time with the electrophysiological setup. The characteristics of this model are first estimated using white noise current injection. The electrode and membrane contribution are digitally separated, and the recording is then made by online subtraction of the electrode contribution. Tests performed in vitro and in vivo demonstrate that AEC enables high-frequency recordings in demanding conditions, such as injection of conductance noise in dynamic-clamp mode, not feasible with a single high-resistance electrode until now. AEC should be particularly useful to characterize fast neuronal phenomena intracellularly in vivo.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2008.06.021