Albinism and cell viability in cycloartenol synthase deficient Arabidopsis

Phenotypes of Arabidopsis thaliana that carry mutations in CYCLOARTENOL SYNTHASE 1 (CAS1) which is required in sterol biosynthesis have been described. Knockout mutant alleles are responsible of a male-specific transmission defect. Plants carrying a weak mutant allele cas1-1 accumulate 2,3-oxidosqua...

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Bibliographic Details
Published in:Plant signaling & behavior 2008-11, Vol.3 (11), p.978-980
Main Authors: Babiychuk, Elena, Navé, Pierrette Bouvier, Compagnon, Vincent, Suzuki, Masashi, Muranaka, Toshiya, Van Montagu, Marc, Kushnir, Sergei, Schaller, Hubert
Format: Article
Language:English
Subjects:
Addendum
albino
alleles
Arabidopsis thaliana
Binding
Biology
Bioscience
biosynthesis
Calcium
Cancer
carotenoids
Cell
cell viability
Cellular Biology
chlorophyll
chloroplasts
Cycle
isomerases
Landes
leaves
Life Sciences
mutants
mutation
Organogenesis
photoperiod
phytosterols
pigments
Proteins
seedlings
shoots
stems
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Summary:Phenotypes of Arabidopsis thaliana that carry mutations in CYCLOARTENOL SYNTHASE 1 (CAS1) which is required in sterol biosynthesis have been described. Knockout mutant alleles are responsible of a male-specific transmission defect. Plants carrying a weak mutant allele cas1-1 accumulate 2,3-oxidosqualene, the substrate of CAS1, in all analyzed organs. Mutant cas1-1 plants develop albino inflorescence shoots that contain low amount of carotenoids and chlorophylls. The extent of this albinism, which affects Arabidopsis stems late in development, may be modulated by the light/dark regime. The fact that chloroplast differentiation and pigment accumulation in inflorescence shoots are associated with a low CAS1 expression could suggest the involvement of 2,3-oxidosqualene in a yet unknown regulatory mechanism linking the sterol biosynthetic segment, located in the cytoplasm, and the chlorophyll and carotenoid biosynthetic segments, located in the plastids, in the highly complex terpenoid network. CAS1 loss of function in a mosaic analysis of seedlings further demonstrated that leaf albinism associated with an accumulation of 2,3-oxidosqualene is a novel phenotype for plant sterol deficient mutant. Addendum to: Babiychuk E, Bouvier-Navé P, Compagnon V, Suzuki M, Muranaka T, Van Montagu M, Kushnir S, Schaller H. Allelic mutant series reveal distinct functions for Arabidopsis cycloartenol synthase 1 in cell viability and plastid biogenesis. Proc Natl Acad Sci USA 2008; 105:3163-8.
ISSN:1559-2316
1559-2324
1559-2324
DOI:10.4161/psb.6173