Keratinolytic proteases of Bacillus species isolated from the Amazon basin showing remarkable de-hairing activity

Three keratinolytic Bacillus spp. isolated from the Brazilian Amazon basin were characterized. The strains P6, P7 and P11 were identified based on morphological and biochemical characteristics and 16S rDNA sequences. P6, P7 and P11 sequences shared more than 99% similarity with B. subtilis, B. amylo...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2007-03, Vol.23 (3), p.375-382
Main Authors: Giongo, Janice Luhering, Lucas, Françoise S, Casarin, Franciani, Heeb, Philipp, Brandelli, Adriano
Format: Article
Language:English
Subjects:
Bacillus
Bacillus (bacteria)
Bacteria
Biochemistry
Biodiversity
Biological and medical sciences
Biotechnology
Depilatory activity
Ecology, environment
Epidermis
feathers
Fundamental and applied biological sciences. Psychology
Keratinase
leather
Life Sciences
Microbiology
Microscopic analysis
Protease
Proteinase inhibitors
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Summary:Three keratinolytic Bacillus spp. isolated from the Brazilian Amazon basin were characterized. The strains P6, P7 and P11 were identified based on morphological and biochemical characteristics and 16S rDNA sequences. P6, P7 and P11 sequences shared more than 99% similarity with B. subtilis, B. amyloliquefaciens and B. velesensis. The keratinases produced by these bacteria were active on azokeratin and degradation of feather barbules was observed. The enzymes were inhibited by the serine protease inhibitor PMSF, and showed maximum activity at pH 9.0. Proteins like albumin, casein and gelatin were hydrolysed by these keratinases. Depilatory studies on bovine pelts revealed that all three strains were efficient in promoting de-hairing. Microscopic analysis showed that the epidermis was completely removed and the absence of hair in follicles was observed.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-006-9234-1