Recurrent recovery of Pseudomonas oryzihabitans strains in a karstified chalk aquifer

Pseudomonas oryzihabitans is an uncommon pathogen that may cause catheter-associated infections, particularly in immunocompromised patients. Although it has been isolated from environment, the source of human infection is not well documented. In the present study, 14 isolates of P. oryzihabitans wer...

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Bibliographic Details
Published in:Water research (Oxford) 2007-01, Vol.41 (1), p.111-117
Main Authors: Dussart-Baptista, L., Bodilis, J., Barray, S., Frébourg, N., Fournier, M., Dupont, J.-P., Jouenne, T.
Format: Article
Language:English
Subjects:
Aluminum Silicates - chemistry
animal pathogens
Aquifer
biofilm
Biofilms
Calcium Carbonate - chemistry
Continental interfaces, environment
DNA fingerprinting
groundwater contamination
Heterotrophic Processes
Karst
karsts
microbial contamination
microbial genetics
Microscopy, Electron, Scanning
molecular sequence data
nucleotide sequences
Polymorphism, Genetic
Pseudomonas - genetics
Pseudomonas - isolation & purification
Pseudomonas - pathogenicity
Pseudomonas - physiology
Pseudomonas oryzihabitans
random amplified polymorphic DNA technique
ribosomal RNA
Sciences of the Universe
suspended particulate matter
Suspended particulate matters
Water Purification
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Summary:Pseudomonas oryzihabitans is an uncommon pathogen that may cause catheter-associated infections, particularly in immunocompromised patients. Although it has been isolated from environment, the source of human infection is not well documented. In the present study, 14 isolates of P. oryzihabitans were recovered over a 28-month period from a karstified chalk aquifer, allowing to advance that distributed natural water could be a source of contamination. Microbiological analyses showed that the bacterium was mainly associated with suspended particulate matters. To investigate the clonality of P. oryzihabitans environmental isolates, 16S rRNA gene sequencing, antibiogram and randomly amplified polymorphic DNA (RAPD) typings were performed. Results demonstrated (i) the presence of at least three clones within the aquifer and (ii) that the presence of the bacterium in groundwater is not only the result of a biofilm bloom but also of an exogenous contamination.
ISSN:0043-1354
1879-2448
DOI:10.1016/j.watres.2006.10.007