Detection of Mycoplasma synoviae in poultry environment samples by culture and polymerase chain reaction

Successful detection of Mycoplasma synoviae (MS) by culture and PCR from samples collected in the environment of experimentally infected chickens and turkeys, or under field conditions, is described. Results showed that in the experimental infection, 10/96 and 46/96 samples of food, drinking water,...

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Bibliographic Details
Published in:Veterinary microbiology 2000-05, Vol.73 (4), p.311-318
Main Authors: Marois, Corinne, Oufour-Gesbert, Fabienne, Kempf, Isabelle
Format: Article
Language:English
Subjects:
Animals
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Chicken-bacteria
Chickens
Chickens - microbiology
Culture
Diagnosis-bacteria
DNA Primers
DNA Primers - chemistry
DNA, Bacterial
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
Dust
Electrophoresis, Agar Gel
Electrophoresis, Agar Gel - veterinary
Environment
Feathers
Feathers - microbiology
Feces
Feces - microbiology
Food Microbiology
Fundamental and applied biological sciences. Psychology
Life Sciences
Microbiology
Microbiology and Parasitology
Mycoplasma
Mycoplasma - chemistry
Mycoplasma - genetics
Mycoplasma - isolation & purification
Mycoplasma Infections
Mycoplasma Infections - microbiology
Mycoplasma Infections - transmission
Mycoplasma Infections - veterinary
Mycoplasma synoviae
PCR
Polymerase Chain Reaction
Polymerase Chain Reaction - veterinary
Poultry Diseases
Poultry Diseases - microbiology
Poultry Diseases - transmission
Specific Pathogen-Free Organisms
Trachea
Trachea - microbiology
Turkeys
Turkeys - microbiology
Water Microbiology
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Summary:Successful detection of Mycoplasma synoviae (MS) by culture and PCR from samples collected in the environment of experimentally infected chickens and turkeys, or under field conditions, is described. Results showed that in the experimental infection, 10/96 and 46/96 samples of food, drinking water, feathers, droppings or dust were positive by culture and Mycoplasma-PCR. In field conditions, the number of positive results for environmental samples were respectively 7/28 and 17/28. These observations highlight the high disseminating capacities of this mycoplasma and show the usefulness of the PCR method for epidemiological studies.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(00)00178-4