Chiral Stationary Phase Based on a Biostable l-RNA Aptamer

An immobilized anti-l-arginine d-RNA aptamer, used as a target-specific chiral stationary phase (CSP), was found to be very quickly degraded by RNases under usual chromatographic utilization and storage. To overcome this severe limitation for a practical use, a CSP based on the l-RNA aptamer, that i...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-04, Vol.77 (7), p.1993-1998
Main Authors: Brumbt, Agnès, Ravelet, Corinne, Grosset, Catherine, Ravel, Anne, Villet, Annick, Peyrin, Eric
Format: Article
Language:English
Subjects:
Analytical chemistry
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Arginine - isolation & purification
Arginine - metabolism
Bacterial Proteins - chemistry
Biotin - analogs & derivatives
Biotin - chemistry
Chemistry
Chemistry Techniques, Analytical - instrumentation
Chemistry Techniques, Analytical - methods
Chromatographic methods and physical methods associated with chromatography
Chromatography
Enzymes
Exact sciences and technology
Life Sciences
Other chromatographic methods
Pharmaceutical sciences
Ribonucleases - metabolism
Ribonucleic acid
RNA
RNA Stability
Stereoisomerism
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Summary:An immobilized anti-l-arginine d-RNA aptamer, used as a target-specific chiral stationary phase (CSP), was found to be very quickly degraded by RNases under usual chromatographic utilization and storage. To overcome this severe limitation for a practical use, a CSP based on the l-RNA aptamer, that is, the mirror image of the d-RNA aptamer, was created. It was shown that this mirror-image approach was a very simple and powerful strategy to develop a highly stable stationary phase due to the intrinsic insensitivity of l-RNA to the RNase degradation. In addition, such an approach allowed one to reverse the enantiomer elution order relative to that obtained with the corresponding d-RNA CSP.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac048344l