Ancylobacter dichloromethanicus sp. nov. – a new aerobic facultatively methylotrophic bacterium utilizing dichloromethane

• Published in: Systematic and applied microbiology 2009-07, Vol.32 (4), p.227-232
• Main Authors: Firsova, Julia, Doronina, Nina, Lang, Elke, Spröer, Cathrin, Vuilleumier, Stéphane, Trotsenko, Yuri
• Format: Article
• Language: English
• Subjects: Aerobic facultative methylotroph; Alphaproteobacteria - classification; Alphaproteobacteria - isolation & purification; Alphaproteobacteria - metabolism; Alphaproteobacteria - ultrastructure; Bacteria; Base Sequence; Biochemistry, Molecular Biology; Biodegradation, Environmental; Dichloromethane degrader; Gram-Negative Aerobic Rods and Cocci - classification; Gram-Negative Aerobic Rods and Cocci - isolation & purification; Gram-Negative Aerobic Rods and Cocci - metabolism; Gram-Negative Aerobic Rods and Cocci - ultrastructure; Life Sciences; Methylene Chloride - metabolism; Molecular Sequence Data; Phylogeny; Ribulose bisphosphate pathway; Soil Microbiology; Soil Pollutants - metabolism; Taxonomy
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2009.02.002

A novel aerobic facultative methylotroph was isolated from contaminated soil. The organism (strain DM16) is a Gram-negative asporogenous non-motile curved rod multiplying by binary fission. Cells are neutrophilic and mesophilic. This strain utilized dichloromethane, methanol, formate and formaldehyde along with a variety of polycarbon compounds. Strain DM16 employs the ribulosebisphosphate pathway for C 1 assimilation. The DNA G+C content is 64.5 mol%. The major ubiquinone is Q-10. The dominant cellular fatty acids are 18:1ω7c (58.6%), cyclo-19:0ω8c (34.8%) and 16:0 (3.2%). Sequencing of the 16S rRNA gene and DNA–DNA hybridization experiments clearly indicated that this methylotroph should be classified as a new species within genus Ancylobacter – Ancylobacter dichloromethanicus sp. nov. with the type strain DM16 T (DSM 21507 T=VKM B-2484 T).