Photoelectrochemical Immunosensor for Label-Free Detection and Quantification of Anti-cholera Toxin Antibody

We demonstrate herein a newly developed photoelectrochemical immunosensor for the determination of anti-cholera toxin antibody by using a photosensitive biotinylated polypyrrole film. The latter was generated by electro-oxidation of a biotinylated tris(bipyridyl) ruthenium(II) complex bearing pyrrol...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-08, Vol.128 (30), p.9693-9698
Main Authors: Haddour, Naoufel, Chauvin, Jérôme, Gondran, Chantal, Cosnier, Serge
Format: Article
Language:English
Subjects:
Analytical chemistry
Antibodies - chemistry
Antibodies - immunology
Antibody Affinity
Avidin - chemistry
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Chemical Sciences
Cholera Toxin - immunology
Electrochemistry
Fundamental and applied biological sciences. Psychology
Membranes, Artificial
Methods. Procedures. Technologies
Molecular Structure
Organometallic Compounds - chemistry
Photochemistry
Polymers
Ruthenium - chemistry
Surface Properties
Various methods and equipments
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Summary:We demonstrate herein a newly developed photoelectrochemical immunosensor for the determination of anti-cholera toxin antibody by using a photosensitive biotinylated polypyrrole film. The latter was generated by electro-oxidation of a biotinylated tris(bipyridyl) ruthenium(II) complex bearing pyrrole groups. The photoexcitation of this modified electrode potentiostated at 0.5 V vs SCE, in the presence of an oxidative quencher, pentaaminechloro cobalt(III) chloride (15 mM), led to a cathodic photocurrent. As a result of the affinity interactions, a layer of biotinylated cholera toxin was firmly bound to the functionalized polypyrrole film via avidin bridges. The resulting modified electrodes were tested as immunosensors for the detection of the corresponding antibody from 0 to 200 μg mL-1. The antibody concentration was measured through the decrease in photocurrent intensity resulting from its specific binding onto the polymeric coating, the detection limit being 0.5 μg mL-1.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja062729z