Biotin Sulfone as a New Tool for Synthetic Oligosaccharide Immobilization: Application to Multiple Analysis Profiling and Surface Plasmonic Analysis of Anti-Candida albicans Antibody Reactivity against α and β (1→2) Oligomannosides

• Published in: Journal of medicinal chemistry 2008-10, Vol.51 (19), p.6201-6210
• Main Authors: Collot, Mayeul, Sendid, Boualem, Fievez, Aurélie, Savaux, Camille, Standaert-Vitse, Annie, Tabouret, Marc, Drucbert, Anne Sophie, Marie Danzé, Pierre, Poulain, Daniel, Mallet, Jean-Maurice
• Format: Article
• Language: English
• Subjects: Antibodies, Fungal - analysis; Antibodies, Fungal - chemistry; Avidin - chemistry; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biotin - analogs & derivatives; Biotin - chemistry; Candida albicans - immunology; Carbohydrate Conformation; Carbohydrate Sequence; Chemical Sciences; Immunoassay; Molecular Sequence Data; Oligosaccharides - chemical synthesis; Oligosaccharides - chemistry; Organic chemistry; Sensitivity and Specificity; Sulfones - chemistry; Surface Plasmon Resonance - instrumentation; Surface Plasmon Resonance - methods; Surface Properties
• ISSN: 0022-2623; 1520-4804
• DOI: 10.1021/jm800099g

As a part of our glycoantigen synthetic program for diagnosis and basic analysis of yeast-related pathogenic mechanisms, a library of 1→2 oligomannosides suitable for immunoanalysis was prepared. The use of biotin sulfone, an oxidized form of biotin, offers a convenient solution for both oligosaccharide synthesis and immobilization on microspheres and surface plasmon resonance sensors. The application of this new strategy for the analysis of anti-Candida albicans antibody response through multiple-analyte profiling technology (Luminex) and with surface plasmonic analysis using biotin tagged synthetic oligosaccharides on avidin coated surfaces was validated using monoclonal antibodies.