Two distinct classes of FixJ binding sites defined by in vitro selection

The phosphorylated FixJ transcriptional activator is key to nitrogen fixation in Sinorhizobium meliloti, switching both the nifA and fixK genes on. Previously no consensus picture emerged concerning the nature of FixJ binding sites. Here we used in vitro DNA selection in order to systematically char...

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Bibliographic Details
Published in:FEBS letters 2002-04, Vol.517 (1), p.185-189
Main Authors: Ferrières, Lionel, Kahn, Daniel
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence - physiology
Binding site
Binding Sites
DNA, Bacterial - analysis
FixJ
Life Sciences
Molecular Sequence Data
Nitrogen fixation
Nitrogen Fixation - physiology
Other
Promoter Regions, Genetic - physiology
Sinorhizobium meliloti - genetics
Sinorhizobium meliloti - metabolism
Trans-Activators - metabolism
Transcriptional regulator
Two-component system
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Summary:The phosphorylated FixJ transcriptional activator is key to nitrogen fixation in Sinorhizobium meliloti, switching both the nifA and fixK genes on. Previously no consensus picture emerged concerning the nature of FixJ binding sites. Here we used in vitro DNA selection in order to systematically characterise FixJ binding sequences. This led to the definition of two classes of sites. Class I sites share the CTAAGTAGTTTCCC sequence found in the fixK promoter, whereas class II sites are defined by a GTAMGTAG consensus octamer. Our results indicate that FixJ∼P binds DNA following two distinct binding modes.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)02618-2