Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness

Two membrane electrochemical reactors (MER) were designed and applied to HLADH-catalysed reduction of cyclohexanone to cyclohexanol. The regeneration of the cofactor NADH was ensured electrochemically, using either methyl viologen or a rhodium complex as electrochemical mediator. A semi-permeable me...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2004-07, Vol.26 (4), p.205-215
Main Authors: Délécouls-Servat, K, Bergel, A, Basséguy, R
Format: Article
Language:English
Subjects:
Alcohol Dehydrogenase - chemistry
Bioreactors
Catalysis
Coenzymes - chemistry
Cyclohexanols - chemical synthesis
cyclohexanones
Cyclohexanones - chemistry
Dialysis
Electrochemistry
Electrochemistry - instrumentation
Electrochemistry - methods
electrodes
Enzyme Activation
Enzymes, Immobilized - chemistry
Equipment Design
Equipment Failure Analysis
Filtration
Life Sciences
membranes
Membranes, Artificial
Microbiology and Parasitology
NAD (coenzyme)
NAD - chemistry
Oxidation-Reduction
paraquat
Reactors
Recycling
Rhodium
Ultrafiltration - methods
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Summary:Two membrane electrochemical reactors (MER) were designed and applied to HLADH-catalysed reduction of cyclohexanone to cyclohexanol. The regeneration of the cofactor NADH was ensured electrochemically, using either methyl viologen or a rhodium complex as electrochemical mediator. A semi-permeable membrane (dialysis or ultra-filtration) was integrated in the filter-press electrochemical reactor to confine the enzyme(s) as close as possible to the electrode surface. When methyl viologen was used, the transformation ratio of cyclohexanone varied from 0 to 65% depending on the internal arrangement of the reactor. Matching the reactor configuration to the reaction system was essential in this case. With the rhodium complex, the ultra-filtration MER was tested in continuous and recycling configurations. The best conditions led to 100% transformation of 0.1 L volume of 0.1 M cyclohexanone after 70 h with the recycling mode. Finally, the performances of the reactors are discussed with respect to different evaluations of the production yields.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-004-0356-2