Sequence diversity of the OprD protein of environmental Pseudomonas strains

OprD has been widely described for Pseudomonas aeruginosa at both structural and functional levels. Here, we describe the sequence diversity of the OprD proteins from other fluorescent Pseudomonads. We analysed the sequence of the oprD gene in each of the 49 Pseudomonas isolates, mostly putida and f...

Full description

Saved in:
Bibliographic Details
Published in:Environmental microbiology 2007-03, Vol.9 (3), p.824-835
Main Authors: Chevalier, Sylvie, Bodilis, Josselin, Jaouen, Thomas, Barray, Sylvie, Feuilloley, Marc G.J, Orange, Nicole
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Continental interfaces, environment
DNA, Bacterial - analysis
Environmental Microbiology
Environmental Sciences
Genetic Variation
Global Changes
Hospitals
Molecular Sequence Data
Plant Roots - microbiology
Porins - genetics
Pseudomonas - classification
Pseudomonas - genetics
Pseudomonas - growth & development
Pseudomonas - isolation & purification
Pseudomonas aeruginosa
Pseudomonas aeruginosa - classification
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - growth & development
Pseudomonas aeruginosa - isolation & purification
Sciences of the Universe
Sequence Analysis, DNA
Soil Microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:OprD has been widely described for Pseudomonas aeruginosa at both structural and functional levels. Here, we describe the sequence diversity of the OprD proteins from other fluorescent Pseudomonads. We analysed the sequence of the oprD gene in each of the 49 Pseudomonas isolates, mostly putida and fluorescens species, obtained from various environmental sources, including soil, rhizosphere and hospitals. Phylogeny based on OprD sequences distinguished three well-separated clusters in the P. fluorescens species whereas P. putida isolates formed only one cluster. The OprD sequences were generally well conserved within each cluster whereas on the opposite, they were highly variable from one cluster to another and particularly with regards to the cluster of P. aeruginosa. Predicted secondary structures, based on the topological model elaborated for P. aeruginosa, suggest signatures in the large extracellular loops of OprD, which are linked to the OprD-based clusters. Correlations between these OprD-based clusters and ecological niches, growth on various carbon sources and antibiotic sensitivity were investigated.
ISSN:1462-2912
1462-2920
DOI:10.1111/j.1462-2920.2006.01191.x