Anion conductance of the human red cell is carried by a maxi-anion channel

Historically, the anion transport through the human red cell membrane has been perceived to be mediated by Band 3, in the two-component concept with the large electroneutral anion exchange accompanied by the conductance proper, which dominated the total membrane conductance. The status of anion chan...

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Bibliographic Details
Published in:Blood cells, molecules, & diseases molecules, & diseases, 2010-04, Vol.44 (4), p.243-251
Main Authors: Glogowska, Edyta, Dyrda, Agnieszka, Cueff, Anne, Bouyer, Guillaume, Egée, Stéphane, Bennekou, Poul, Thomas, Serge L.Y.
Format: Article
Language:English
Subjects:
Anion Exchange Protein 1, Erythrocyte - physiology
Biochemistry, Molecular Biology
Chloride Channels - blood
Chloride Channels - physiology
Chlorides - blood
Culture Media, Serum-Free - pharmacology
Erythrocytes - metabolism
Human red blood cell
Humans
Ion Channel Gating
Life Sciences
Maxi-anion channels
Membrane
Nitrobenzoates - pharmacology
Patch-Clamp Techniques
Serum
Thiocyanates - metabolism
Thiocyanates - pharmacology
Up-Regulation
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Summary:Historically, the anion transport through the human red cell membrane has been perceived to be mediated by Band 3, in the two-component concept with the large electroneutral anion exchange accompanied by the conductance proper, which dominated the total membrane conductance. The status of anion channels proper has never been clarified, and the informations obtained by different groups of electrophysiologists are rather badly matched. This study, using the cell-attached configuration of the patch-clamp technique, rationalizes and explains earlier confusing results by demonstrating that the diversity of anionic channel activities recorded in human erythrocytes corresponds to different kinetic modalities of a unique type of maxi-anion channel with multiple conductance levels and probably multiple gating properties and pharmacology, depending on conditions. It demonstrates the role of activator played by serum in the recruitment of multiple new conductance levels showing very complex kinetics and gating properties upon serum addition. These channels, which seem to be dormant under normal physiological conditions, are potentially activable and could confer a far higher anion conductance to the red cell than the ground leak mediated by Band 3.
ISSN:1079-9796
1096-0961
DOI:10.1016/j.bcmd.2010.02.014