Tobacco mutants with reduced microtubule dynamics are less susceptible to TMV

A panel of seven SR1 tobacco mutants (ATER1 to ATER7) derived via T-DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2010-06, Vol.62 (5), p.829-839
Main Authors: Ouko, Maurice O, Sambade, Adrian, Brandner, Katrin, Niehl, Annette, Peña, Eduardo, Ahad, Abdul, Heinlein, Manfred, Nick, Peter
Format: Article
Language:English
Subjects:
activation tagging
Biological and medical sciences
Cellular Biology
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Life Sciences
microtubules
Microtubules - metabolism
movement protein
Mutation
Nicotiana - genetics
Nicotiana - virology
Nicotiana tabacum SR1:nn cv. 'Petit Havana'
Phenylcarbamates - pharmacology
Plant diseases
Plant Diseases - genetics
Plant Leaves - genetics
Plant Leaves - virology
Plant physiology and development
Plant Viral Movement Proteins - metabolism
Tobacco
Tobacco mosaic virus
Tobacco Mosaic Virus - physiology
Tubulin - metabolism
Urethane - pharmacology
Virus Replication
Viruses
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Summary:A panel of seven SR1 tobacco mutants (ATER1 to ATER7) derived via T-DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these lines, ATER2, α-tubulin is shifted from the tyrosinylated into the detyrosinated form, and the microtubule plus-end marker GFP-EB1 moves significantly slower when expressed in the background of the ATER2 mutant as compared with the SR1 wild type. The efficiency of cell-to-cell movement of TMV encoding GFP-tagged movement protein (MP-GFP) is reduced in ATER2 accompanied by a reduced association of MP-GFP with plasmodesmata. This mutant is also more tolerant to viral infection as compared with the SR1 wild type, implying that reduced microtubule dynamics confer a comparative advantage in face of TMV infection.
ISSN:0960-7412
1365-313X
DOI:10.1111/j.1365-313X.2010.04195.x