Cyanines as New Fluorescent Probes for DNA Detection and Two-Photon Excited Bioimaging

A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding towa...

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Bibliographic Details
Published in:Organic letters 2010-05, Vol.12 (10), p.2194-2197
Main Authors: Feng, Xin Jiang, Wu, Po Lam, Bolze, Frédéric, Leung, Heidi W. C, Li, King Fai, Mak, Nai Ki, Kwong, Daniel W. J, Nicoud, Jean-François, Cheah, Kok Wai, Wong, Man Shing
Format: Article
Language:English
Subjects:
Animals
Biochemistry, Molecular Biology
Cattle
Cellular Biology
DNA - analysis
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Life Sciences
Microscopy, Fluorescence, Multiphoton - methods
Nitriles - chemical synthesis
Nitriles - chemistry
Photons
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Summary:A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol100474b