Endothelin-1 as a Mediator of Endothelial Cell-Pericyte Interactions in Bovine Brain Capillaries

Endothelial cells and pericytes are closely associated in brain capillaries. Together with astrocytic foot processes, they form the blood–brain barrier. Capillaries were isolated from bovine brain cortex. Pure populations of endothelial cells and pericytes were isolated and cultured in vitro. Polari...

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Bibliographic Details
Published in:Journal of cerebral blood flow and metabolism 1997-04, Vol.17 (4), p.464-469
Main Authors: Dehouck, Marie-Pierre, Vigne, Paul, Torpier, Gérard, Breittmayer, Jean Philippe, Cecchelli, Roméo, Frelin, Christian
Format: Article
Language:English
Subjects:
Animals
Biochemistry, Molecular Biology
Biological and medical sciences
Blood-Brain Barrier
Capillaries - cytology
Cattle
Cell Communication
Cell Polarity
Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges
Cerebrovascular Circulation
Coculture Techniques
Endothelin Receptor Antagonists
Endothelin-1 - biosynthesis
Endothelin-1 - physiology
Endothelium, Vascular - cytology
Endothelium, Vascular - metabolism
Fundamental and applied biological sciences. Psychology
Life Sciences
Peptides, Cyclic - pharmacology
Receptors, Endothelin - metabolism
Vertebrates: nervous system and sense organs
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Summary:Endothelial cells and pericytes are closely associated in brain capillaries. Together with astrocytic foot processes, they form the blood–brain barrier. Capillaries were isolated from bovine brain cortex. Pure populations of endothelial cells and pericytes were isolated and cultured in vitro. Polarized monolayers of endothelial cells preferentially secreted immunoreactive endothelin-1 (Et-1) at their abluminal (brain-facing) membrane. They did not express receptors for Et-1. Pericytes expressed BQ-123-sensitive ETA receptors for endothelins as evidenced by 125I-Et-1 binding experiments. These receptors were coupled to phospholipase C as demonstrated by intracellular calcium measurements using indo-1-loaded cells. Addition of Et-1 to pericytes induced marked changes in the cell morphology that were associated with a reorganization of F-actin and intermediate filaments. It is concluded that Et-1 is a paracrine mediator at the bovine blood–brain barrier and that capillary pericytes are target cells for endothelium-derived Et-1.
ISSN:0271-678X
1559-7016
DOI:10.1097/00004647-199704000-00012