Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle

Skeletal muscle cells constitute a heterogeneous population that maintains muscle integrity through a high myogenic regenerative capacity. More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in se...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2010-12, Vol.28 (12), p.2182-2194
Main Authors: Pisani, Didier F., Clement, Noémie, Loubat, Agnès, Plaisant, Magali, Sacconi, Sabrina, Kurzenne, Jean‐Yves, Desnuelle, Claude, Dani, Christian, Dechesne, Claude A.
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - metabolism
Adipogenesis
Adolescent
Adult
Aged
Antigens, CD - metabolism
Biopsy
CD34
CD56 Antigen - metabolism
Cell Differentiation
Cell Lineage
Child
Child, Preschool
Clone Cells
Development Biology
Humans
Infant
Life Sciences
Middle Aged
Models, Biological
Multipotential differentiation
Muscle Cells - cytology
Muscle Cells - metabolism
Muscle stem cell
Muscle, Skeletal - cytology
Muscle, Skeletal - pathology
Myogenesis
Stem Cells - cytology
Stem Cells - metabolism
Young Adult
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Summary:Skeletal muscle cells constitute a heterogeneous population that maintains muscle integrity through a high myogenic regenerative capacity. More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle‐derived cells to decipher the cellular basis of the myoadipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myoadipogenic bipotent progenitors. These progenitors were isolated in the CD34+ population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myoadipogenic CD56+CD15+ progenitors gives rise to myogenic CD56+CD15− progenitors and adipogenic CD56−CD15+ progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. These results provide cellular bases for adipogenic differentiation in human skeletal muscle, which may explain the fat development encountered in different muscle pathological situations. STEM CELLS 2010;28:2182–2194
ISSN:1066-5099
1066-6099
1549-4918
DOI:10.1002/stem.537