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Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle
Skeletal muscle cells constitute a heterogeneous population that maintains muscle integrity through a high myogenic regenerative capacity. More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in se...
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Published in: | Stem cells (Dayton, Ohio) Ohio), 2010-12, Vol.28 (12), p.2182-2194 |
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creator | Pisani, Didier F. Clement, Noémie Loubat, Agnès Plaisant, Magali Sacconi, Sabrina Kurzenne, Jean‐Yves Desnuelle, Claude Dani, Christian Dechesne, Claude A. |
description | Skeletal muscle cells constitute a heterogeneous population that maintains muscle integrity through a high myogenic regenerative capacity. More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle‐derived cells to decipher the cellular basis of the myoadipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myoadipogenic bipotent progenitors. These progenitors were isolated in the CD34+ population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myoadipogenic CD56+CD15+ progenitors gives rise to myogenic CD56+CD15− progenitors and adipogenic CD56−CD15+ progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. These results provide cellular bases for adipogenic differentiation in human skeletal muscle, which may explain the fat development encountered in different muscle pathological situations. STEM CELLS 2010;28:2182–2194 |
doi_str_mv | 10.1002/stem.537 |
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More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle‐derived cells to decipher the cellular basis of the myoadipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myoadipogenic bipotent progenitors. These progenitors were isolated in the CD34+ population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myoadipogenic CD56+CD15+ progenitors gives rise to myogenic CD56+CD15− progenitors and adipogenic CD56−CD15+ progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. These results provide cellular bases for adipogenic differentiation in human skeletal muscle, which may explain the fat development encountered in different muscle pathological situations. 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More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle‐derived cells to decipher the cellular basis of the myoadipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myoadipogenic bipotent progenitors. These progenitors were isolated in the CD34+ population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myoadipogenic CD56+CD15+ progenitors gives rise to myogenic CD56+CD15− progenitors and adipogenic CD56−CD15+ progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. These results provide cellular bases for adipogenic differentiation in human skeletal muscle, which may explain the fat development encountered in different muscle pathological situations. STEM CELLS 2010;28:2182–2194</description><subject>Adipocytes - cytology</subject><subject>Adipocytes - metabolism</subject><subject>Adipogenesis</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens, CD - metabolism</subject><subject>Biopsy</subject><subject>CD34</subject><subject>CD56 Antigen - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Lineage</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Clone Cells</subject><subject>Development Biology</subject><subject>Humans</subject><subject>Infant</subject><subject>Life Sciences</subject><subject>Middle Aged</subject><subject>Models, Biological</subject><subject>Multipotential differentiation</subject><subject>Muscle Cells - cytology</subject><subject>Muscle Cells - metabolism</subject><subject>Muscle stem cell</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - pathology</subject><subject>Myogenesis</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Young Adult</subject><issn>1066-5099</issn><issn>1066-6099</issn><issn>1549-4918</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOGzEUQK2qiEeK1C-oZldYDL22x2N7GSFKkBKBlCCWluO507idR2rPFIWvZ4YEdl3dh47O4hDylcIVBWA_Yof1leDyEzmlItNppqn6POyQ56kArU_IWYy_AWgmlDomJww0zyXkp2Q58xhscBv_YjvfNklbJotd-wsb7xLbFMm08NvD-RDelq4NMXny3cY3yayvbZMs_2CFna2SRR9dhV_IUWmriOeHOSGPP29W17N0fn97dz2dpy4TmUypZRKoK6jkVgsmFC1pxpBxJ3PQUuZWFcjoGtcoUJccLBZl4dQaWaFYRvmEXO69G1uZbfC1DTvTWm9m07kZfwCCcyng38h-37Pb0P7tMXam9tFhVdkG2z4axRjPQWk-kBd70oU2xoDlh5qCGWubsbYZag_ot4O0X9dYfIDveQcg3QPPvsLdf0VmubpZjMJX9OOI1g</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Pisani, Didier F.</creator><creator>Clement, Noémie</creator><creator>Loubat, Agnès</creator><creator>Plaisant, Magali</creator><creator>Sacconi, Sabrina</creator><creator>Kurzenne, Jean‐Yves</creator><creator>Desnuelle, Claude</creator><creator>Dani, Christian</creator><creator>Dechesne, Claude A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Alphamed Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2020-5831</orcidid><orcidid>https://orcid.org/0000-0001-5879-8527</orcidid><orcidid>https://orcid.org/0000-0003-3228-0230</orcidid></search><sort><creationdate>201012</creationdate><title>Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle</title><author>Pisani, Didier F. ; 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More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle‐derived cells to decipher the cellular basis of the myoadipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myoadipogenic bipotent progenitors. These progenitors were isolated in the CD34+ population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myoadipogenic CD56+CD15+ progenitors gives rise to myogenic CD56+CD15− progenitors and adipogenic CD56−CD15+ progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. 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subjects | Adipocytes - cytology Adipocytes - metabolism Adipogenesis Adolescent Adult Aged Antigens, CD - metabolism Biopsy CD34 CD56 Antigen - metabolism Cell Differentiation Cell Lineage Child Child, Preschool Clone Cells Development Biology Humans Infant Life Sciences Middle Aged Models, Biological Multipotential differentiation Muscle Cells - cytology Muscle Cells - metabolism Muscle stem cell Muscle, Skeletal - cytology Muscle, Skeletal - pathology Myogenesis Stem Cells - cytology Stem Cells - metabolism Young Adult |
title | Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle |
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