novel Hansenula polymorpha transcriptional factor HpHAP4‐B, able to functionally replace the S. cerevisiae HAP4 gene, contains an additional bZip motif

The transcriptional regulator HAP4, whose expression is induced on respiratory substrates, has been shown to be involved in the balance between fermentation and respiration in Saccharomyces cerevisiae. We have previously identified a HAP4 orthologue in the yeast Hansenula polymorpha, called HpHAP4‐A...

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Published in:Yeast (Chichester, England) England), 2010-11, Vol.27 (11), p.941-954
Main Authors: Sybirna, K, Petryk, N, Zhou, Y.‐F, Sibirny, A, Bolotin‐Fukuhara, M
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Biochemistry, Molecular Biology
bZip
CCAAT box
CCAAT-Binding Factor - deficiency
fermentation
genes
Genetic Complementation Test
H. polymorpha
Hansenula polymorpha
Life Sciences
Microbiology and Parasitology
Molecular Sequence Data
Pichia - genetics
Protein Binding
regulation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
transcription factor
transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
yeasts
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Summary:The transcriptional regulator HAP4, whose expression is induced on respiratory substrates, has been shown to be involved in the balance between fermentation and respiration in Saccharomyces cerevisiae. We have previously identified a HAP4 orthologue in the yeast Hansenula polymorpha, called HpHAP4‐A, which, despite its very limited sequence conservation (a 16 amino acid N‐terminal motif), is fully functional in S. cerevisiae. Based on the same N‐terminal motif, a second gene has now been identified in the same organism. It was shown to contain an additional cis‐binding motif of the bZip type. We report on the cloning, heterologous expression and analysis in S. cerevisiae of this novel ScHAP4 orthologue. From these experiments we could conclude that, as with HpHAP4‐A, the novel orthologue, designated HpHAP4‐B, could functionally replace the S. cerevisiae gene but to a lesser extent. The relationship between the presence of the additional cis‐binding motif and the weaker potential as a HAP4 functional homologue is discussed. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
1097-0061
DOI:10.1002/yea.1802