Heteronemin, a spongean sesterterpene, inhibits TNFα-induced NF-κB activation through proteasome inhibition and induces apoptotic cell death

• Published in: Biochemical pharmacology 2010-02, Vol.79 (4), p.610-622
• Main Authors: Schumacher, Marc, Cerella, Claudia, Eifes, Serge, Chateauvieux, Sébastien, Morceau, Franck, Jaspars, Marcel, Dicato, Mario, Diederich, Marc
• Format: Article
• Language: English
• Subjects: Animals; Anti-cancer drug discovery; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Apoptosis - physiology; Humans; Jurkat Cells; K562 Cells; Marine natural product; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; NF-κB; Porifera - chemistry; Proteasome Endopeptidase Complex - physiology; Proteasome Inhibitors; Sesterterpenes - chemistry; Sesterterpenes - isolation & purification; Sesterterpenes - pharmacology; Terpenes - chemistry; Terpenes - isolation & purification; Terpenes - pharmacology; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 0006-2952; 1873-2968
• DOI: 10.1016/j.bcp.2009.09.027

In this study, we investigated the biological effects of heteronemin, a marine sesterterpene isolated from the sponge Hyrtios sp. on chronic myelogenous leukemia cells. To gain further insight into the molecular mechanisms triggered by this compound, we initially performed DNA microarray profiling and determined which genes respond to heteronemin stimulation in TNFα-treated cells and which genes display an interaction effect between heteronemin and TNFα. Within the differentially regulated genes, we found that heteronemin was affecting cellular processes including cell cycle, apoptosis, mitogen-activated protein kinases (MAPKs) pathway and the nuclear factor κB (NF-κB) signaling cascade. We confirmed in silico experiments regarding NF-κB inhibition by reporter gene analysis, electrophoretic mobility shift analysis and I-κB degradation. In order to assess the underlying molecular mechanisms, we determined that heteronemin inhibits both trypsin and chymotrypsin-like proteasome activity at an IC 50 of 0.4 μM. Concomitant to the inhibition of the NF-κB pathway, we also observed a reduction in cellular viability. Heteronemin induces apoptosis as shown by annexin V-FITC/propidium iodide-staining, nuclear morphology analysis, pro-caspase-3, -8 and -9 and poly(ADP-ribose) polymerase (PARP) cleavage as well as truncation of Bid. Altogether, results show that this compound has potential as anti-inflammatory and anti-cancer agent.