Love wave biosensor for real-time detection of okadaic acid as DSP phycotoxin

Love wave acoustic delay lines were used as sensors for okadaic acid immuno-detection through immobilized specific antibodies. Anti-okadaic acid antibodies were grafted onto the sensor surface. Antibodies, saturating agent, and target species, okadaic acid (test line) or an irrelevant peptide (contr...

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Bibliographic Details
Main Authors: Fournel, F., Baco, E., Mamani-Matsuda, M., Degueil, M., Bennetau, B., Moynet, D., Mossalayi, D., Vellutini, L., Pillot, J.-P., Dejous, C., Rebiere, D.
Format: Conference Proceeding
Language:English
Subjects:
Acoustic wave
Biodetection
Engineering Sciences
Micro and nanotechnologies
Microelectronics
Microfluidic
Phycotoxin
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Summary:Love wave acoustic delay lines were used as sensors for okadaic acid immuno-detection through immobilized specific antibodies. Anti-okadaic acid antibodies were grafted onto the sensor surface. Antibodies, saturating agent, and target species, okadaic acid (test line) or an irrelevant peptide (control line), were injected through an optimized microfluidic chip. Similarly to sandwich ELISA, polyclonal antibodies were then injected a second time to bind to the acid. They led to frequency shifts three times superior with okadaic acid than with the peptide.
ISSN:1877-7058
1877-7058
DOI:10.1016/j.proeng.2010.09.237