Biochemical alterations in juvenile carp ( Cyprinus carpio) exposed to zinc: Glutathione reductase as a target

The aim of this study was to investigate biochemical changes in juvenile carp ( Cyprinus carpio) exposed to zinc chloride (10, 30 and 100 μM) for a period of 48 h. Zinc exposure caused a concentration-dependent reduction in glutathione reductase (GR) activity in gills, liver and brain. Gill glutathi...

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Published in:Marine environmental research 2008-07, Vol.66 (1), p.88-89
Main Authors: Franco, Jeferson L., Posser, Thais, Mattos, Jacó J., Sánchez-Chardi, Alejandro, Trevisan, Rafael, Oliveira, Camila S., Carvalho, Paulo S.M., Leal, Rodrigo B., Marques, Maria R.F., Bainy, Afonso C.D., Dafre, Alcir L.
Format: Article
Language:English
Subjects:
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Brain - drug effects
Brain - enzymology
Carps - metabolism
Chlorides - toxicity
Cyprinus carpio
Ecotoxicology, biological effects of pollution
Freshwater
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
Gills - drug effects
Gills - enzymology
Glutathione Reductase - metabolism
Glutathione reductase inhibition
HSP60
HSP70
Liver - drug effects
Liver - enzymology
MAPK
Marine
p38
p38 Mitogen-Activated Protein Kinases - genetics
Phosphorylation - drug effects
Sea water ecosystems
Synecology
Techniques
Zinc
Zinc Compounds - toxicity
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Summary:The aim of this study was to investigate biochemical changes in juvenile carp ( Cyprinus carpio) exposed to zinc chloride (10, 30 and 100 μM) for a period of 48 h. Zinc exposure caused a concentration-dependent reduction in glutathione reductase (GR) activity in gills, liver and brain. Gill glutathione S-transferase (GST) was reduced when animals were exposed to the highest concentration of 100 μM zinc. The phosphorylation of p38 MAPK increased in the brain of fish exposed to zinc 100 μM, while phosphorylation of the extracellular signal-regulated protein kinase 1/2 (ERK1/2) and c-Jun N-terminal protein kinase 1/2 (JNK1/2) remained unchanged. Expression of proteins HSP60 and HSP70 were not affected by zinc exposure. Considering the significant concentration-dependent inhibition of GR in all tissues analyzed, this enzyme could be a potential biomarker of exposure to zinc, which has to be confirmed.
ISSN:0141-1136
1879-0291
DOI:10.1016/j.marenvres.2008.02.031