Distribution of genovars and the new variant of in the Göteborg area, Sweden

• Published in: European journal of clinical microbiology & infectious diseases 2010-03, Vol.29 (5), p.609-611
• Main Authors: Lagergård, T., Hadad, R., Tunbäck, P., Lindholm, L., Löwhagen, G.-B., Unemo, M.
• Format: Article
• Language: English
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s10096-010-0887-1

The aims of this study were to assess the proportion of the new variant of (nvCT) and the distribution of genovars among -positive patients in the Göteborg area, Sweden. Consecutive urine samples positive for using BD ProbeTec ET (177 patients, 88 men and 89 women) were collected. An nvCT-specific real-time polymerase chain reaction (PCR) assay was used to investigate the nvCT prevalence. To identify the genovars, a 990-bp DNA segment from 105 specimens was sequenced. Seventeen percent (30/177) of all specimens contained nvCT. Nine different genovars were identified. About 50% were of genovar E, followed by F 16%, G 11%, K 8%, and D 5%, representing about 90% of the specimens in Göteborg. The occurrence of nvCT and the dominance of genovar E in Göteborg is similar to those in other areas of Sweden. To cover about 90% of the infections in Sweden, the serovars D, E, F, G, and K should be included in future vaccines based on the major outer membrane protein.