Serodiagnosis of infections in pigs

The aim of this study is the development and evaluation of a serodiagnostic assay for (MA). After screening MA lipid fractions in an ELISA format, a polar lipid fraction was selected as antigen because of its superior recognition by serum antibodies in experimentally infected pigs. The resulting MA-...

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Published in:Veterinary microbiology 2010-04, Vol.142 (3-4)
Main Authors: Wisselink, Henk J., van Solt-Smits, Conny B., Oorburg, Derk, van Soolingen, Dick, Overduin, Pieter, Maneschijn-Bonsing, Judith, Stockhofe-Zurwieden, Norbert, Buys-Bergen, Herma, Engel, Bas, Urlings, Bert A.P., Thole, Jelle E.R.
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container_issue 3-4
container_start_page
container_title Veterinary microbiology
container_volume 142
creator Wisselink, Henk J.
van Solt-Smits, Conny B.
Oorburg, Derk
van Soolingen, Dick
Overduin, Pieter
Maneschijn-Bonsing, Judith
Stockhofe-Zurwieden, Norbert
Buys-Bergen, Herma
Engel, Bas
Urlings, Bert A.P.
Thole, Jelle E.R.
description The aim of this study is the development and evaluation of a serodiagnostic assay for (MA). After screening MA lipid fractions in an ELISA format, a polar lipid fraction was selected as antigen because of its superior recognition by serum antibodies in experimentally infected pigs. The resulting MA-ELISA was evaluated as an alternative for detection of MA infection by traditional pathological examination of pig lymph nodes for granulomatous lesions by meat inspectors. By comparing with bacteriological examination, the MA-ELISA showed significantly better sensitivity (69%) as compared to pathological examination (31%) in experimentally infected pigs. The MA-ELISA also appeared significantly more specific in a set of serum samples from MA negative pigs: only 1 out of these 153 serum samples reacted positive, whereas 99 (65%) of these had displayed false positive results by detection of lymph nodes lesions that appeared not to be associated with MA (Komijn et al., 2007).
doi_str_mv 10.1016/j.vetmic.2009.11.003
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title Serodiagnosis of infections in pigs
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