Urinary signature of anabolic steroids and glucocorticoids in humans by LC–MS

A metabonomic strategy based on LC–MS was employed to investigate the metabolic profile of urine samples from 20 athletes who had been tested positive for corticoids and anabolic steroids and 29 controls. In this aim, different sample preparations and chromatographic conditions were compared. The ac...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 2011-02, Vol.83 (5), p.1769-1773
Main Authors: Kiss, Agneta, Jacquet, Anne-Laure, Paisse, Olivier, Flament-Waton, Marie-Magdeleine, de Ceaurriz, Jacques, Bordes, Claire, Gauvrit, Jean-Yves, Lantéri, Pierre, Cren-Olivé, Cécile
Format: Article
Language:English
Subjects:
Anabolic Agents - urine
Anabolics
Analytical chemistry
Chemical Sciences
Chemistry
Chromatography, Liquid - methods
Corticoids
Doping products
Exact sciences and technology
Glucocorticoids - urine
Humans
LC–MS
Metabolic profiling
Metabolomics
Metabonomics
Steroids - urine
Tandem Mass Spectrometry - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A metabonomic strategy based on LC–MS was employed to investigate the metabolic profile of urine samples from 20 athletes who had been tested positive for corticoids and anabolic steroids and 29 controls. In this aim, different sample preparations and chromatographic conditions were compared. The acquired LC–MS data of doped athletes and controls were subjected to analysis of variance (ANOVA) and principal component analysis (PCA). Using this approach, molecular signature of human urine was obtained showing that metabonomics could be a complementary tool to discriminate different urinary profiles and to track down metabolic changes in humans.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2010.10.030