Foslip® -based photodynamic therapy as a means to improve wound healing

• Published in: Photodiagnosis and photodynamic therapy 2011-12, Vol.8 (4), p.321-327
• Main Authors: Garrier, Julie, Bezdetnaya, Lina, Barlier, Catherine, Gräfe, Susanna, Guillemin, François, D’Hallewin, Marie-Ange
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry, Molecular Biology; Biophysics; Collagen; Female; Foslip; Hematology, Oncology and Palliative Medicine; Internal Medicine; Life Sciences; Mesoporphyrins - therapeutic use; Mice; Mice, Nude; Photochemotherapy - methods; Photodynamic therapy; Photosensitizing Agents - therapeutic use; Treatment Outcome; Wound healing; Wound Healing - drug effects; Wound Healing - radiation effects; Wounds, Penetrating - drug therapy; Wounds, Penetrating - pathology
• ISSN: 1572-1000; 1873-1597
• DOI: 10.1016/j.pdpdt.2011.06.003

Summary Background Collagen matrices as substitution for connective tissue are known to promote wound healing. Photodynamic therapy has been anecdotally associated with improved wound healing and reduced scarring. The present study investigates the impact of collagen based scaffolding material, embedded with a liposomal formulation of meta-tetra (hydroxyphenyl) chlorin (mTHPC, Foslip® ) and photodynamic therapy on wound healing in mice. Methods After incision in the neck region, two different types of collagen material, previously incubated with Foslip® at different concentrations, were implanted followed by illumination at 652 nm (10 J/cm2 , 100 mW/cm2 ). Mice were imaged daily up to two weeks, whereafter excision was performed and pathological analysis. Results Scab detachment was observed at day seven for controls whereas it occurred as early as three days for PDT at the lowest concentrations. In the latter conditions, final matrix remodelling could be observed as evidenced by elastin neosynthesis. Conclusions Topical application of low dose Foslip® in a collagen matrix followed by illumination considerably accelerates wound healing.