Reduced N cycling in response to drought, warming, and elevated CO2 in a Danish heathland: Synthesizing results of the CLIMAITE project after two years of treatments

• Published in: Global change biology 2010-11, Vol.17 (5)
• Main Authors: Larsen, Klaus Steenberg, Andresen, Louise C., Beier, Claus, Jonasson, Sven, Albert, Kristian R., Ambus, Per, Stevnbak, Karen, Arndal, Marie Frost, Carter, Mette S., Christensen, Soren, Holmstrup, Martin, Ibrom, Andreas, Kongstad, Jane, van Der Linden, Leon, Maraldo, Kristine, Michelsen, Anders, Mikkelsen, Teis Nørgaard, Pilegaard, Kim, Prieme, Ambers, Ro-Poulsen, Helge, Schmidt, Inger K. Kappel, Selsted, Merete Bang
• Format: Article
• Language: English
• ISSN: 1354-1013; 1365-2486
• DOI: 10.1111/j.1365-2486.2010.02351.x

Field-scale experiments simulating realistic future climate scenarios are important tools for investigating the effects of current and future climate changes on ecosystem functioning and biogeochemical cycling. We exposed a semi-natural Danish heathland ecosystem to elevated atmospheric carbon dioxide (CO2), warming, and extended summer drought in all combinations. Here, we report on the short-term responses of the nitrogen (N) cycle after two years of treatments. Elevated CO2 significantly affected above-ground stoichiometry by increasing the carbon to nitrogen (C/N) ratios in the leaves of both co-dominant species (Calluna vulgaris and Deschampsia flexuosa), as well as the C/N ratios of Calluna flowers and by reducing the N concentration of Deschampsia litter. Below-ground, elevated CO2 had only minor effects, whereas warming increased N turnover, as indicated by increased rates of microbial NH4+-N consumption, gross mineralization, potential nitrification, denitrification and N2O emissions. Drought reduced below-ground gross N mineralization and decreased fauna N mass and N mineralization. Leaching was unaffected by treatments but was significantly higher across all treatments in the second year than in the much drier first year indicating that ecosystem N loss is highly sensitive to changes and variability in amount and timing of precipitation. Interactions between treatments were common and although some synergistic effects were observed, antagonism dominated the interactive responses in treatment combinations, i.e. responses were smaller in combinations than in single treatments. Nonetheless, increased C/N ratios of photosynthetic tissue, decreased litter N production, and decreased fauna N mineralization prevailed in the full treatment combination indicating reduced N turnover in the simulated future climate scenario, which could act to reduce the potential growth response of plants to elevated atmospheric CO2 concentration. In conclusion, effects observed in single-factor studies should be evaluated with caution. Multi-factor climate experiments are needed for improving realistic estimation of the combined ecosystem responses to future climate changes.