A randomised trial to investigate the effects of acute consumption of a blackcurrant juice drink on markers of vascular reactivity and bioavailability of anthocyanins in human subjects

• Published in: European journal of clinical nutrition 2011-07, Vol.65 (7), p.849-856
• Main Authors: Jin, Y, Alimbetov, D, George, T, Gordon, M H, Lovegrove, J A
• Format: Article
• Language: English
• Subjects: 631/443/592; 631/92/436/1729; 692/699/75; Adult; Anthocyanin; Anthocyanins; Anthocyanins - analysis; Anthocyanins - metabolism; Anthocyanins - therapeutic use; Anthocyanins - urine; Antioxidants; Antioxidants - analysis; Ascorbic acid; Beverages - analysis; Bioavailability; Biological and medical sciences; Biological Availability; Biomarkers; Biomarkers - blood; Blood Vessels - drug effects; Blood Vessels - physiology; Cardiovascular diseases; Cardiovascular Diseases - epidemiology; Cardiovascular Diseases - prevention & control; Care and treatment; Clinical Nutrition; Consumption; Cross-Over Studies; Diagnosis; Diet; Dietary intake; Doppler effect; Double-Blind Method; Epidemiology; Feeding. Feeding behavior; Female; Flavonoids; Food intake; Fruit - chemistry; Fruit juices; Fundamental and applied biological sciences. Psychology; Glycosides; Health aspects; Health risks; Humans; Intercellular Adhesion Molecule-1 - blood; Internal Medicine; Juices; Lasers; Lipids; Low concentrations; Male; Medical sciences; Medicine; Medicine & Public Health; Metabolic Diseases; Metabolites; Microorganisms; Middle Aged; Nutrient deficiency; Nutrition research; Nutritive Value; original-article; Phenols - blood; Phenols - urine; Plasma; Public Health; Reactivity; Ribes - chemistry; Risk analysis; Risk Factors; United Kingdom - epidemiology; Urine; Vascular Cell Adhesion Molecule-1 - blood; Veins & arteries; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/ejcn.2011.55

Background/Objectives: To study the bioavailability of anthocyanins and the effects of a 20% blackcurrant juice drink on vascular reactivity, plasma antioxidant status and other cardiovascular disease risk markers. Subjects/Methods: The study was a randomised, cross-over, double-blind, placebo-controlled acute meal study. Twenty healthy volunteers (11 females and 9 males) were recruited, and all subjects completed the study. Fasted volunteers consumed a 20% blackcurrant juice drink (250 ml) or a control drink following a low-flavonoid diet for the previous 72 h. Vascular reactivity was assessed at baseline and 120 min after juice consumption by laser Doppler imaging (LDI). Plasma and urine samples were collected periodically over an 8-h period for analysis, with a final urine sample collected at 24 h. The cross-over was performed after a 4-week washout. Results: There were no significant effects of the 20% blackcurrant juice drink on acute measures of vascular reactivity, biomarkers of endothelial function or lipid risk factors. Consumption of the test juice caused increases in plasma vitamin C ( P= 0.006), and urinary anthocyanins ( P