single step method for purification of sulfated oligosaccharides

Purifying and analysing sulfated oligosaccharides by mass spectrometry often constitutes a challenge. We present here a single step method to isolate sulfated compounds from a complex mixture of neutral and acidic oligosaccharide-alditols. The strategy relies on the exclusion of sulfated molecules f...

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Bibliographic Details
Published in:Glycoconjugate journal 2008-12, Vol.25 (9), p.903-915
Main Authors: Garenaux, Estelle, Yu, Shin-Yi, Florea, Doina, Strecker, Gérard, Khoo, Kay-Hooi, Guérardel, Yann
Format: Article
Language:English
Subjects:
Amphibians
Animals
Biochemistry
Biochemistry - methods
Biochemistry, Molecular Biology
Biomedical and Life Sciences
Biophysics
Bronchi
Carbohydrate Conformation
Carbohydrate Sequence
Chemical Fractionation
Chromatography, Ion Exchange
Humans
Life Sciences
Mass spectrometry
Molecular biology
Molecular Sequence Data
mucins
Mucins - chemistry
Oligosaccharides - chemistry
Oligosaccharides - isolation & purification
Pathology
Proteins
purification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sugar Alcohols - chemistry
Sulfated oligosaccharides
Sulfates - chemistry
Sulfates - isolation & purification
Trachea
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Summary:Purifying and analysing sulfated oligosaccharides by mass spectrometry often constitutes a challenge. We present here a single step method to isolate sulfated compounds from a complex mixture of neutral and acidic oligosaccharide-alditols. The strategy relies on the exclusion of sulfated molecules from strong cation exchange resin. By testing a wide range of reduced mucin type O-glycans isolated from different biological sources, we demonstrate that this method permits, without prior chemical modification, the specific purification of sulfate-containing oligosaccharides present in any quantity from very complex mixtures of molecules.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-008-9139-3