TWO-DIMENSIONAL GEL ELECTROPHORESIS ANALYSIS OF BROWN ALGAL PROTEIN EXTRACTS

High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protei...

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Bibliographic Details
Published in:Journal of phycology 2008-10, Vol.44 (5), p.1315-1321
Main Authors: Contreras, Loretto, Ritter, Andrés, Dennett, Geraldine, Boehmwald, Freddy, Guitton, Nathalie, Pineau, Charles, Moenne, Alejandra, Potin, Philippe, Correa, Juan A
Format: Article
Language:English
Subjects:
2-DE
Algae
Ecology, environment
Ectocarpus siliculosus
Life Sciences
Marine
Phaeophyceae
protein extraction methods
proteomics
Scytosiphon gracilis
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Summary:High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protein extracts. The performance of the new protocol was tested against four methods available for vascular plants and a seaweed. The protocol, which includes an initial step to remove salts from the algal tissues, allowed the use of highly resolving two-dimensional gel electrophoresis (2-DE) protein analyses, providing the opportunity to unravel potentially novel physiological processes unique to this group of marine organisms.
ISSN:0022-3646
1529-8817
DOI:10.1111/j.1529-8817.2008.00575.x