Dark-field digital holographic microscopy for 3D-tracking of gold nanoparticles

We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample with a localization accuracy independent of the particle posit...

Full description

Saved in:
Bibliographic Details
Published in:Optics express 2011-12, Vol.19 (27), p.26044-26055
Main Authors: Verpillat, F, Joud, F, Desbiolles, P, Gross, M
Format: Article
Language:English
Subjects:
Equipment Design
Equipment Failure Analysis
Gold - isolation & purification
Holography - instrumentation
Imaging, Three-Dimensional - instrumentation
Microscopy - instrumentation
Molecular Imaging - instrumentation
Nanoparticles - ultrastructure
Optics
Physics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample with a localization accuracy independent of the particle position. From our measurements we reconstruct the trajectories of the particles and derive their 3D diffusion coefficient. Our results pave the way for quantitative studies of the motion of single nanoparticle in complex media.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.19.026044