3D exploration of light scattering from live cells in the presence of gold nanomarkers using holographic microscopy

In this paper, we explore the 3D structure of light scattering from dark-field illuminated live 3T3 cells in the presence of 40 nm gold nanomarkers. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and the phase-shifting acquisition of the...

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Bibliographic Details
Published in:3D research 2011-03, Vol.2 (1), Article 2
Main Authors: Joud, Fadwa, Warnasooriya, Nilanthi, Bun, Philippe, Verpillat, Frédéric, Suck, Sarah Y., Tessier, Gilles, Atlan, Michael, Desbiolles, Pierre, Coppey-Moisan, Maïté, Abboud, Marie, Gross, Michel
Format: Article
Language:English
Subjects:
3DR Express
Computer Imaging
Engineering
Lasers
Optical Devices
Optics
Pattern Recognition and Graphics
Photonics
Physics
Signal,Image and Speech Processing
Vision
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Summary:In this paper, we explore the 3D structure of light scattering from dark-field illuminated live 3T3 cells in the presence of 40 nm gold nanomarkers. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and the phase-shifting acquisition of the digital holograms. A comparative study of the 3D reconstructions of the scattered fields allows us to locate the gold markers which yield, contrarily to the cell structures, well defined bright scattering patterns that are not angularly titled and clearly located along the optical axis (z). This characterization is an unambiguous signature of the presence of gold biological nanomarkers, and validates the capability of digital holographic microscopy to discriminate them from background signals in live cells.
ISSN:2092-6731
2092-6731
DOI:10.1007/3DRes.01(2011)2