Flow cytometric measurement of the clearance rate in the blue mussel Mytilus edulis and the development of a new individual exposure system for aquatic immunotoxicological studies

Animals in poor health condition are not relevant biological models. The current study focused on the use of the clearance rate of Mytilus edulis to assess the gross physiological condition of individuals maintained in stressful experimental conditions. This approach was developed in a new, highly c...

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Bibliographic Details
Published in:Environmental pollution (1987) 2008-05, Vol.153 (2), p.492-496
Main Authors: Duchemin, Matthieu B., Wessel, Nathalie, Fournier, Michel, Auffret, Michel
Format: Article
Language:English
Subjects:
animal health
Animal, plant and microbial ecology
Animals
Applied ecology
Aquatic pollution
Biological and medical sciences
Clearance rate
Dose-Response Relationship, Drug
Ecotoxicology - methods
Ecotoxicology, biological effects of pollution
endpoints
Environmental Exposure
Environmental Monitoring - methods
Environmental Sciences
experimental design
Flow Cytometry
Fundamental and applied biological sciences. Psychology
General aspects
Immune parameters
immune response
immunotoxicity
in tubo studies
in vitro studies
Individual exposure
Invertebrates
laboratory techniques
Metabolic Clearance Rate
Mollusca
mussels
Mytilus edulis
Mytilus edulis - drug effects
Mytilus edulis - metabolism
new methods
subacute toxicity
Water Pollutants, Chemical - pharmacokinetics
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Summary:Animals in poor health condition are not relevant biological models. The current study focused on the use of the clearance rate of Mytilus edulis to assess the gross physiological condition of individuals maintained in stressful experimental conditions. This approach was developed in a new, highly controlled experimental exposure device designed to investigate individual responses in aquatic ecotoxicological studies. Both clearance rate values and immune parameters analysis indicated that the health condition of mussels kept in 50 ml tubes for 24 h or 48 h was not altered compared to controls, while most parameters were depressed after 72 h. Moreover, this study confirms the relevance of flow cytometric for the measurement of clearance rate compared to techniques utilizing microscopy. Current results prompted us to perform further 24 h chemical exposure using this “in tubo” device. Test-tube mussels offer a new exposure design for immunotoxicology.
ISSN:0269-7491
1873-6424
DOI:10.1016/j.envpol.2007.08.017