Capillary zone electrophoresis as a potential technique for the simultaneous determination of sulfadoxine and pyrimethamine in tablet formulations

A novel, simple and rapid capillary zone electrophoresis method with UV detection has been developed for the simultaneous determination of pyrimethamine and sulfadoxine in tablet formulations. The compounds are separated in 6 min in a fused silica capillary, 30 cm long (20 cm to detector) x 50 μm us...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2012, Vol.58, p.168-171
Main Authors: Blanchin, Marie-Dominique, Aké, Michèle, Fabre, Huguette, Amin, N'Cho Christophe
Format: Article
Language:English
Subjects:
Chemical Sciences
Organic chemistry
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Summary:A novel, simple and rapid capillary zone electrophoresis method with UV detection has been developed for the simultaneous determination of pyrimethamine and sulfadoxine in tablet formulations. The compounds are separated in 6 min in a fused silica capillary, 30 cm long (20 cm to detector) x 50 μm using a 100 mM phosphate buffer pH 7.2 as background electrolyte, a 330 V cm--1 electric field and a detection wavelength of 214 nm. Analysis of different tablet formulations has shown a good agreement with the liquid chromatography method described in the United States Pharmacopoeia. Main advantages of the CZE method are the rapid set-up of instrumentation and capillary equilibration, short analysis time and low running cost.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.pba.2011.09.028