Characterization of a 50-Ω Exposure Setup for High-Voltage Nanosecond Pulsed Electric Field Bioexperiments

An exposure system for a nanosecond pulsed electric field is presented and completely characterized in this paper. It is composed of a high-voltage generator and an applicator: the biological cuvette. The applied pulses have high intensities (up to 5 kV), short durations (3 and 10 ns), and different...

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Bibliographic Details
Published in:IEEE transactions on biomedical engineering 2011-01, Vol.58 (1), p.207-214
Main Authors: Kenaan, Mohamad, Amari, Saad El, Silve, Aude, Merla, Caterina, Mir, Lluis M., Couderc, Vincent, Arnaud-Cormos, Delia, Leveque, Philippe
Format: Article
Language:English
Subjects:
Bandwidth
Biological and medical sciences
Biological cuvette matching bandwidth
Biology
Coaxial cables
Computer Simulation
Electric fields
Electric Impedance
Electric potential
Electrodes
Electromagnetic Fields
Electronics
Electroporation - methods
Engineering Sciences
finite-difference time domain (FDTD)
Fundamental and applied biological sciences. Psychology
General aspects
Generators
high voltage (HV)
High voltages
Homogeneity
Impedance
Mathematical analysis
Mathematical models
Nanosecond pulsed electric field (nsPEF)
Nanostructure
Transmission line measurements
Voltage
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Summary:An exposure system for a nanosecond pulsed electric field is presented and completely characterized in this paper. It is composed of a high-voltage generator and an applicator: the biological cuvette. The applied pulses have high intensities (up to 5 kV), short durations (3 and 10 ns), and different shapes (square, bipolar). A frequency characterization of the cuvette is carried out based on both an analytical model and experimental measurements (S 11 ) in order to determine its matching bandwidth. High voltage measurements in the time domain are performed. Results show that the cuvette is well adapted to 10-ns pulses and limited to those of 3 ns. The rise/fall times of the pulses should not be less than 1.5 ns. In addition, numerical calculation providing voltage distribution within the cuvette is performed using an in-house finite-difference time-domain code. A good level of voltage homogeneity across the cuvette electrodes is obtained, as well as consistency with experimental data for all the applied pulses.
ISSN:0018-9294
1558-2531
DOI:10.1109/TBME.2010.2081670