Intramyocardial protein therapy with vascular endothelial growth factor (VEGF-165) induces functional angiogenesis in rat senescent myocardium

Myocardial capillary density and angiogenesis are impaired during aging but whether growth factor therapy is able to induce functional neovascularization in senescent heart have never been studied. In 3, 24, 28 and 32 mo male Wistar rats, cardiac hemodynamic measurements indicated heart failure at 2...

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Published in:Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 2010-12, Vol.61 (6), p.651-61
Main Authors: Besse, Sophie, Boucher, François, Linguet, Gilberte, Riou, Laurent, de Leiris, Joël, Riou, Bertrand, Dimastromatteo, Julien, Comte, René, Courty, José, Delbe, Jean
Format: Article
Language:English
Subjects:
Age Factors
Animals
Coronary Vessels
Down-Regulation
Heart Failure
Human health and pathology
Humans
Hypertrophy, Left Ventricular
Life Sciences
Male
Myocardium
Neovascularization, Physiologic
Rats
Rats, Wistar
Recombinant Proteins
Tissues and Organs
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-2
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Summary:Myocardial capillary density and angiogenesis are impaired during aging but whether growth factor therapy is able to induce functional neovascularization in senescent heart have never been studied. In 3, 24, 28 and 32 mo male Wistar rats, cardiac hemodynamic measurements indicated heart failure at 28 and 32 mo, associated with left ventricular hypertrophy. VEGF/VEGF-R2, Ang-1/Ang-2/Tie-2 and PTN levels, quantitated in left ventricle by western blotting and immunohistochemistry, showed that VEGF and VEGF-R2 levels were specifically decreased during aging. In vitro angiogenesis ± rhVEGF-165 (5 and 50 ng/mL) was measured in aortic segments in 3D-collagen. Aortic sprouting was decreased during aging but restored by VEGF treatment (P
ISSN:0867-5910