Beet necrotic yellow vein virus subgenomic RNA3 is a cleavage product leading to stable non-coding RNA required for long-distance movement

Beet necrotic yellow vein virus (BNYVV) is a multipartite RNA virus. BNYVV RNA3 does not accumulate in non-host transgenic Arabidopsis thaliana plants when expressed using a 35S promoter. However, a 3'-derivative species has been detected in transgenic plants and in transient expression assays...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 2012-05, Vol.93 (Pt 5), p.1093-1102
Main Authors: PELTIER, Claire, KLEIN, Elodie, HLEIBIEH, Kamal, D'ALONZO, Massimiliano, HAMMANN, Philippe, BOUZOUBAA, Salah, RATTI, Claudio, GILMER, David
Format: Article
Language:English
Subjects:
Arabidopsis - virology
Beta vulgaris - virology
Biochemistry, Molecular Biology
Biological and medical sciences
Cellular Biology
Fundamental and applied biological sciences. Psychology
Genome, Viral
Life Sciences
Microbiology
Miscellaneous
Molecular biology
Nicotiana - virology
Phytopathology. Animal pests. Plant and forest protection
Plant Viruses - genetics
Plant Viruses - pathogenicity
Plant viruses and viroids
RNA Stability
RNA, Untranslated - metabolism
RNA, Viral - genetics
RNA, Viral - metabolism
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Beet necrotic yellow vein virus (BNYVV) is a multipartite RNA virus. BNYVV RNA3 does not accumulate in non-host transgenic Arabidopsis thaliana plants when expressed using a 35S promoter. However, a 3'-derivative species has been detected in transgenic plants and in transient expression assays conducted in Nicotiana benthamiana and Beta macrocarpa. The 3'-derivative species is similar to the previously reported subgenomic RNA3 produced during virus infection. 5' RACE revealed that the truncated forms had identical 5' ends. The 5' termini carried the coremin motif also present on BNYVV RNA5, beet soil-borne mosaic virus RNA3 and 4, and cucumber mosaic virus group 2 RNAs. This RNA3 species lacks a m(7)Gppp at the 5' end of the cleavage products, whether expressed transiently or virally. Mutagenesis revealed the importance of the coremin sequence for both long-distance movement and stabilization of the cleavage product in vivo and in vitro. The isolation of various RNA3 5'-end products suggests the existence of a cleavage between nt 212 and 1234 and subsequent exonucleolytic degradation, leading to the accumulation of a non-coding RNA. When RNA3 was incubated in wheatgerm extracts, truncated forms appeared rapidly and their appearance was protein- and divalent ion-dependent.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.039685-0