Fluorescence-lifetime imaging with a multifocal two-photon microscope

Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for fluorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the l...

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Bibliographic Details
Published in:Optics letters 2004-12, Vol.29 (24), p.2884-2886
Main Authors: LEVEQUE-FORT, S, FONTAINE-AUPART, M. P, ROGER, G, GEORGES, P
Format: Article
Language:English
Subjects:
Equipment Design
Equipment Failure Analysis
Exact sciences and technology
Feasibility Studies
Fundamental areas of phenomenology (including applications)
Image Enhancement - instrumentation
Image Enhancement - methods
Image forming and processing
Image Interpretation, Computer-Assisted - instrumentation
Image Interpretation, Computer-Assisted - methods
Imaging and optical processing
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Nonlinear optics
Optics
Physics
Reproducibility of Results
Sensitivity and Specificity
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Summary:Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for fluorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the location of fluorophores but do not identify the physicochemical properties and interactions between cells' components. To protect biological samples from experiments that are too long and to provide a more comprehensive spectroscopic tool we have developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while retaining the possibility of measuring both intensity and lifetime images of the sample.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.29.002884