Herpes simplex virus type 1-based amplicon vectors for fundamental research in neurosciences and gene therapy of neurological diseases

• Published in: Journal of physiology, Paris Paris, 2012-01, Vol.106 (1-2), p.2-11
• Main Authors: Jerusalinsky, Diana, Baez, María Verónica, Epstein, Alberto Luis
• Format: Article
• Language: English
• Subjects: Amplicons; Animals; Anxiety; Ataxia; Biochemistry, Molecular Biology; Brain; Cell culture; Chromosomes; Data processing; Epithelial cells; Experimental gene therapy; Expression vectors; Gene therapy; Gene transfer; Gene Transfer Techniques; Genetic Therapy - methods; Genetic Vectors - physiology; Genomes; Glial cells; Herpes simplex virus 1; Herpesvirus 1, Human - genetics; HSV-1-derived vectors; Humans; Immune response; insertional mutagenesis; Learning; Life Sciences; Memory; Molecular biology; Nervous system; Nervous System Diseases - therapy; Neurodegenerative diseases; Neurodegenerative disorders; Neurological diseases; Neurons; Neurosciences - methods; Nucleotide sequence; Plasticity (neural); Regulatory sequences; Reviews; Toxicity; Transgenes; Tropism
• ISSN: 0928-4257; 1769-7115
• DOI: 10.1016/j.jphysparis.2011.11.003

► HSV-1 based amplicons are very versatile, powerful and promising gene transfer tools. ► They are neurotropic vectors very well suited to deliver genes to the CNS and PNS. ► It is possible nowadays to produce helper-free non-toxic amplicon stocks. ► They are used to deliver large DNA fragments to mammalian cell nucleus. ► They are being used in experimental gene therapy and basic research in neuroscience. Somatic manipulation of the nervous system without the involvement of the germinal line appears as a powerful counterpart of the transgenic strategy. The use of viral vectors to produce specific, transient and localized knockout, knockdown, ectopic expression or overexpression of a gene, leads to the possibility of analyzing both in vitro and in vivo molecular basis of neural function. In this approach, viral particles engineered to carry transgenic sequences are delivered into discrete brain regions, to transduce cells that will express the transgenic products. Amplicons are replication-incompetent helper-dependent vectors derived from herpes simplex virus type 1 (HSV-1), with several advantages that potentiate their use in neurosciences: (1) minimal toxicity: amplicons do not encode any virus proteins, are neither toxic for the infected cells nor pathogenic for the inoculated animals and elicit low levels of adaptive immune responses; (2) extensive transgene capacity to carry up to 150-kb of foreign DNA; i.e., entire genes with regulatory sequences could be delivered; (3) widespread cellular tropism: amplicons can experimentally infect several cell types including glial cells, though naturally the virus infects mainly neurons and epithelial cells; (4) since the viral genome does not integrate into cellular chromosomes there is low probability to induce insertional mutagenesis. Recent investigations on gene transfer into the brain using these vectors, have focused on gene therapy of inherited genetic diseases affecting the nervous system, such as ataxias, or on neurodegenerative disorders using experimental models of Parkinson’s or Alzheimer’s disease. Another group of studies used amplicons to investigate complex neural functions such as neuroplasticity, anxiety, learning and memory. In this short review, we summarize recent data supporting the potential of HSV-1 based amplicon vector model for gene delivery and modulation of gene expression in primary cultures of neuronal cells and into the brain of living animals.